Declerck Priscilla, Behets Jonas, van Hoef Vincent, Ollevier Frans
Laboratory of Aquatic Ecology, Department of Biology, Zoological Institute, Katholieke Universiteit Leuven, Charles Deberiotstraat 32, 3000 Leuven, Belgium.
Water Res. 2007 Jul;41(14):3159-67. doi: 10.1016/j.watres.2007.04.011. Epub 2007 Jun 4.
Floating biofilms develop at the water-air interface and harbor numerous microorganisms, some of which are human pathogens like Legionella pneumophila. The presence of Legionella spp. and especially L. pneumophila in such biofilms was investigated. In parallel, the occurrence of Naegleria spp., Acanthamoeba spp., Willaertia spp., Vahlkampfia spp. and Hartmanella spp. was determined and it was examined whether Acanthamoeba spp. isolates were naturally infected with L. pneumophila bacteria. Eight anthropogenic and 37 natural aquatic environments were sampled between June and August 2005. Both Legionella spp. and L. pneumophila were present in 100% of the floating biofilms of the anthropogenic aquatic systems. Eighty-one percent of all natural floating biofilm samples were positive for Legionella spp. and 70% of these samples were positive for L. pneumophila. Legionella concentrations were in the range of 10(1)-10(2)cells/cm(2). Naegleria spp. and Acanthamoeba spp., two well-known L. pneumophila amoeba hosts, were present in 50-92% and 67-72% of floating biofilm samples, respectively. Acanthamoeba spp. isolates appeared to be naturally infected with L. pneumophila bacteria as proved by fluorescent in situ hybridization.
漂浮生物膜在水 - 空气界面形成，其中含有大量微生物，部分微生物是人类病原体，如嗜肺军团菌。对这类生物膜中军团菌属尤其是嗜肺军团菌的存在情况进行了调查。同时，确定了耐格里属、棘阿米巴属、维氏阿米巴属、瓦氏变形虫属和哈特曼氏阿米巴属的出现情况，并检测棘阿米巴属分离株是否自然感染嗜肺军团菌。2月至8月期间对8个人为和37个天然水生环境进行了采样。在人为水生系统的所有漂浮生物膜中均存在军团菌属和嗜肺军团菌。所有天然漂浮生物膜样本中81%军团菌属呈阳性，其中70%的样本嗜肺军团菌呈阳性。军团菌浓度在10(1)-10(2)个细胞/平方厘米范围内。耐格里属和棘阿米巴属这两种著名的嗜肺军团菌变形虫宿主分别存在于50 - 92%和67 - 72%的漂浮生物膜样本中。荧光原位杂交证明，棘阿米巴属分离株似乎自然感染了嗜肺军团菌。
