Su Ping, Henriksson Anders, Mitchell Hazel
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia.
Anaerobe. 2007 Jun-Aug;13(3-4):134-9. doi: 10.1016/j.anaerobe.2007.04.007. Epub 2007 Apr 25.
The aim of the present study was to identify suitable prebiotics for synergistic combinations with representative probiotics from the lactobacilli and bifidobacterium genera. Eleven different types of commercially available carbohydrates and compounds were screened in basal media for their effects on the growth of the probiotic cultures Lactobacillus acidophilus LAFTI L10 (L10), Bifidobacterium animalis lactis LAFTI B94 (B94) and Lactobacillus casei LAFTI L26 (L26). Growth of these probiotics was investigated by measuring optical density (OD) levels over a period of 48 h. The results of this study showed that all three probiotic strains were able to utilize a range of carbohydrates. Maximal growth of L10 was observed in basal medium supplemented with soybean oligosaccharide (SOS), followed by fructooligosaccharide (FOS) and inulin, while maximal growth of B94 was observed in basal medium supplemented with SOS, followed by raffinose, FOS, beta-glucan hydrolysate, inulin and Fibregum TAN. L26 was shown to exhibit maximal growth in basal medium supplemented with FOS followed by inulin, SOS, beta-glucan hydrolysate and beta-glucan concentrate. The results of this study indicate that several carbohydrates/compounds can enhance the growth of these representative probiotic strains in vitro.
本研究的目的是确定适合与乳酸杆菌属和双歧杆菌属代表性益生菌进行协同组合的益生元。在基础培养基中筛选了11种不同类型的市售碳水化合物和化合物，以研究它们对嗜酸乳杆菌LAFTI L10（L10）、动物双歧杆菌乳酸亚种LAFTI B94（B94）和干酪乳杆菌LAFTI L26（L26）等益生菌培养物生长的影响。通过在48小时内测量光密度（OD）水平来研究这些益生菌的生长情况。本研究结果表明，所有三种益生菌菌株都能够利用多种碳水化合物。在添加大豆低聚糖（SOS）的基础培养基中观察到L10的生长达到最大值，其次是低聚果糖（FOS）和菊粉；而在添加SOS的基础培养基中观察到B94的生长达到最大值，其次是棉子糖、FOS、β-葡聚糖水解物、菊粉和Fibregum TAN。结果表明，L26在添加FOS的基础培养基中生长达到最大值，其次是菊粉、SOS、β-葡聚糖水解物和β-葡聚糖浓缩物。本研究结果表明，几种碳水化合物/化合物可以在体外增强这些代表性益生菌菌株的生长。
