Callaghan Innovation Research Limited, PO Box 31-310, Lower Hutt 5040, New Zealand.
Callaghan Innovation Research Limited, PO Box 31-310, Lower Hutt 5040, New Zealand.
Anaerobe. 2014 Feb;25:11-7. doi: 10.1016/j.anaerobe.2013.11.001. Epub 2013 Nov 14.
The utilisation of various prebiotic oligosaccharides by probiotic strains of Bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus acidophilus was investigated in order to determine the synbiotic potential of various prebiotic/probiotic combinations. Analysis by HPLC and high-performance anion-exchange chromatography of the cell-free medium taken during growth of the three probiotic bacteria showed differences in the consumption of the various oligosaccharides. Analysis of galactooligosaccharides showed that both L. rhamnosus and B. lactis consumed mostly mono- and di-saccharide, while L. acidophilus consumed oligosaccharides up to trisaccharide. Both B. lactis and L. acidophilus utilised fructooligosaccharides and inulin, but showed different patterns of oligosaccharide consumption. Only L. rhamnosus grew on β-glucan oligosaccharides and preferentially consumed the trisaccharide. The results indicate the synbiotic potential of the various probiotic/prebiotic combinations, particularly L. acidophilus/galactooligosaccharides, L. acidophilus/fructooligosaccharides or inulin and L. rhamnosus/β-glucan oligosaccharides.
为了确定各种益生元/益生菌组合的共生潜力，研究了双歧杆菌 lactis、鼠李糖乳杆菌和嗜酸乳杆菌的益生菌菌株对各种益生元低聚糖的利用情况。对三种益生菌生长过程中提取的无细胞培养基进行高效液相色谱和高效阴离子交换色谱分析，结果表明各种低聚糖的消耗存在差异。对半乳糖低聚糖的分析表明，L. rhamnosus 和 B. lactis 主要消耗单糖和二糖，而 L. acidophilus 则消耗三糖及以上的低聚糖。B. lactis 和 L. acidophilus 都利用果低聚糖和菊粉，但表现出不同的低聚糖消耗模式。只有 L. rhamnosus 能在 β-葡聚糖低聚糖上生长，并优先消耗三糖。结果表明，各种益生菌/益生元组合具有共生潜力，特别是 L. acidophilus/半乳糖低聚糖、L. acidophilus/果低聚糖或菊粉和 L. rhamnosus/β-葡聚糖低聚糖。
