Patterson A M, Cartwright A, David G, Fitzgerald O, Bresnihan B, Ashton B A, Middleton J
Leopold Muller Arthritis Research Centre, Institute for Science and Technology in Medicine, Medical School, Keele University at Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, UK.
Ann Rheum Dis. 2008 May;67(5):592-601. doi: 10.1136/ard.2006.063875. Epub 2007 Jun 1.
Membrane-bound heparan sulphate proteoglycans (HSPGs) act as co-receptors and presenters of cytokines and are involved in cell-matrix and cell-cell adhesion.
To investigate which HSPGs are expressed in knee joint synovia from patients with different forms of arthritis and normal individuals.
Synovial samples were obtained from patients with early rheumatoid arthritis (n = 8), longstanding rheumatoid arthritis (n = 13), psoriatic arthritis (n = 7), osteoarthritis (n = 6) and normal joints (n = 12). Expression of syndecan-1, -2, -3 and -4 and glypican-1, -3 and -4 was analysed by immunohistochemistry and dual label immunofluorescence.
The expression of HSPGs in chronically inflamed synovium exhibited a differential distribution. Syndecan-1 was present in the mononuclear infiltrates of synovia from patients with rheumatoid and psoriatic arthritis where it was expressed by plasma cells. Syndecan-2 was present mainly in blood vessels where it occurred on endothelial cells, pericytes and smooth muscle cells. Syndecan-3 stained intensely in endothelial cells but also occurred in sublining macrophages and the lining layer. Glypican-4 occurred in the lining layer and blood vessels. Increased expression of these HSPGs was apparent in rheumatoid and psoriatic compared to osteoarthritic and normal synovia. Little or no staining for syndecan-4, glypican-1 and glypican-3 was seen in all samples.
Selected HSPGs, such as syndecan-1, -2 and -3 and glypican-4, could play a part in the pathophysiology of arthritis, such as the migration and retention of leukocytes and angiogenesis in the chronically inflamed synovium.
膜结合型硫酸乙酰肝素蛋白聚糖（HSPGs）作为细胞因子的共受体和呈递者，参与细胞-基质和细胞-细胞黏附。
研究不同类型关节炎患者及正常个体膝关节滑膜中表达的HSPGs。
从早期类风湿关节炎患者（n = 8）、长期类风湿关节炎患者（n = 13）、银屑病关节炎患者（n = 7）、骨关节炎患者（n = 6）和正常关节患者（n = 12）获取滑膜样本。通过免疫组织化学和双标免疫荧光分析syndecan-1、-2、-3和-4以及glypican-1、-3和-4的表达。
HSPGs在慢性炎症滑膜中的表达呈现差异分布。Syndecan-1存在于类风湿关节炎和银屑病关节炎患者滑膜的单核浸润细胞中，由浆细胞表达。Syndecan-2主要存在于血管中，在内皮细胞、周细胞和平滑肌细胞上表达。Syndecan-3在内皮细胞中染色强烈，但也存在于滑膜下层巨噬细胞和衬里层。Glypican-4存在于衬里层和血管中。与骨关节炎和正常滑膜相比，这些HSPGs在类风湿关节炎和银屑病关节炎中的表达明显增加。在所有样本中几乎未见syndecan-4、glypican-1和glypican-3的染色或染色极浅。
选定的HSPGs，如syndecan-1、-2和-3以及glypican-4，可能在关节炎的病理生理学中发挥作用，如在慢性炎症滑膜中白细胞的迁移和滞留以及血管生成。
