König A, Krenn V, Gillitzer R, Glöckner J, Janssen E, Gohlke F, Eulert J, Müller-Hermelink H K
Orthopädische Universitätsklinik König-Ludwig-Haus, Würzburg, Germany.
Rheumatol Int. 1997;17(4):159-68. doi: 10.1007/s002960050028.
Psoriatic arthritis is an inflammatory arthropathy that ultimately can lead to joint destruction. In this study, we investigated the immunophenotypes of the inflammatory cells and the expression of interleukin-8 (IL-8), which is the hallmark chemoattractant cytokine of psoriasis in synovial membranes from patients exhibiting active psoriatic synovitis (n = 9). The tissue samples were examined by immunohistochemistry, Western blot analysis and in situ hybridisation. The inflammatory infiltrate consisted predominantly of CD3+ T lymphocytes, with a higher proportion of CD4+ than CD8+ T lymphocytes in six cases. CD3+ T lymphocytes were focally distributed near small blood vessels and the enlarged synovial intima. CD1+ interdigitating reticulum cells were not detected. CD22+ B lymphocytes and plasma cells were found in small aggregates without KiM4+ follicular dendritic cells. KiM8+ macrophages were located in the synovial intima and were distributed in a diffuse pattern near the synovial lining cells. CD15+ neutrophil granulocytes were detected in four cases. They were preferentially located in the vicinity of blood vessels and the synovial intima. IL-8 was found at a high level in the synovial lining cells and to a lesser extent in cells located in the perivascular areas. Immunofluorescence double staining showed IL-8 to be expressed in KiM8+ multinucleated giant cells, KiM8+ macrophages and CD3+ T lymphocytes. IL-8 receptor A was demonstrated in the synovial lining and in macrophages and lymphocytes. IL-8 was detected by immunoblot analysis of the synovial tissue at 8.4 kD. Employing in situ hybridisation, IL-8 mRNA was strongly and preferentially expressed in the synovial intima, as well as in macrophages and lymphocytes. The immunophenotype of the psoriatic arthritis inflammatory cells shows great similarity to the inflammatory infiltrate found in the synovial tissue of patients with rheumatoid arthritis. The preferential expression of IL-8 and IL-8 mRNA in the enlarged synovial intima and in lymphocytes and macrophages suggests that IL-8 exerts its action through activated mononuclear cells and T lymphocytes. It seems to play a role in regulating leucocyte traffic into the enlarged synovial intima and may contribute to the aggressive synovitis of patients with psoriatic arthritis.
银屑病关节炎是一种最终可导致关节破坏的炎性关节病。在本研究中，我们调查了活动性银屑病滑膜炎患者（n = 9）滑膜中炎性细胞的免疫表型以及白细胞介素-8（IL-8）的表达，IL-8是银屑病的标志性趋化因子细胞因子。通过免疫组织化学、蛋白质印迹分析和原位杂交对组织样本进行检查。炎性浸润主要由CD3 + T淋巴细胞组成，6例中CD4 + T淋巴细胞的比例高于CD8 + T淋巴细胞。CD3 + T淋巴细胞在小血管和增生的滑膜内膜附近呈局灶性分布。未检测到CD1 + 交错突网状细胞。发现CD22 + B淋巴细胞和浆细胞呈小聚集状，无KiM4 + 滤泡树突状细胞。KiM8 + 巨噬细胞位于滑膜内膜，在滑膜衬里细胞附近呈弥漫性分布。4例中检测到CD15 + 中性粒细胞。它们优先位于血管和滑膜内膜附近。在滑膜衬里细胞中发现IL-8水平较高，在血管周围区域的细胞中水平较低。免疫荧光双重染色显示IL-8在KiM8 + 多核巨细胞、KiM8 + 巨噬细胞和CD3 + T淋巴细胞中表达。在滑膜衬里以及巨噬细胞和淋巴细胞中证实有IL-8受体A。通过对滑膜组织的免疫印迹分析在8.4 kD处检测到IL-8。采用原位杂交，IL-8 mRNA在滑膜内膜以及巨噬细胞和淋巴细胞中强烈且优先表达。银屑病关节炎炎性细胞的免疫表型与类风湿关节炎患者滑膜组织中发现的炎性浸润非常相似。IL-8和IL-8 mRNA在增生的滑膜内膜以及淋巴细胞和巨噬细胞中的优先表达表明IL-8通过活化的单核细胞和T淋巴细胞发挥作用。它似乎在调节白细胞向增生的滑膜内膜的迁移中起作用，并且可能导致银屑病关节炎患者的侵袭性滑膜炎。
