Kato M, Ohashi K, Saji F, Wakimoto A, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Placenta. 1991 May-Jun;12(3):217-26. doi: 10.1016/0143-4004(91)90003-x.
The trophoblast cells which contact with maternal tissue express HLA class I either weakly or not at all. This phenomenon is beneficial for the protection of the fetus from immunological rejection by the mother. However, the mechanisms of reduced expression of HLA class I are unexplained. Therefore, we utilized choriocarcinoma cells to analyse the suppressive mechanisms to HLA class I expression in trophoblasts. In this paper we reported the heterogeneity of HLA class I expression in five choriocarcinoma cell lines. The levels of surface HLA class I molecules correlate approximately with mRNA of HLA class I heavy chain, and all choriocarcinoma cells produce moderate amounts of surface and secreted beta 2 microglobulin. It was also found that interferon gamma upregulates HLA class I molecules and mRNA in two choriocarcinoma cell lines, NUC1 and HCCM5.
与母体组织接触的滋养层细胞HLA I类分子表达微弱或完全不表达。这一现象有利于保护胎儿免受母体的免疫排斥。然而，HLA I类分子表达降低的机制尚不清楚。因此，我们利用绒毛膜癌细胞分析滋养层细胞中HLA I类分子表达的抑制机制。本文报道了5种绒毛膜癌细胞系中HLA I类分子表达的异质性。表面HLA I类分子水平与HLA I类重链mRNA大致相关，所有绒毛膜癌细胞均产生中等量的表面和分泌型β2微球蛋白。还发现，干扰素γ可上调两种绒毛膜癌细胞系NUC1和HCCM5中的HLA I类分子和mRNA。
