Watanabe Junji, Akashi Mitsuru
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka, Japan.
Biomacromolecules. 2007 Jul;8(7):2288-93. doi: 10.1021/bm0702915. Epub 2007 Jun 5.
Biomineralization on porous polyester membranes was examined using an improved alternate soaking process (ASP). The effect of ion migration for the formation of hydroxyapatite (HAp) was shown to be crucial. Ion migration was improved by reducing the surface tension by mixing ethanol into an aqueous solution. The resulting hybrid materials were evaluated in terms of calcium content; structure using scanning electron microscopy (SEM), X-ray diffraction (XRD), and infrared spectroscopy (IR); and protein adsorption. The amount of formed HAp was controlled by the number of ASP cycles and also through the ethanol content of the mixed solvent. As the formation of HAp increased, the formed structure could be verified using SEM, IR, and XRD. Protein adsorption was investigated using albumin, gamma-globulin, and fibrinogen, and the amount of adsorbed protein was well-correlated with that of the formed HAp. This result shows that the total amount of the adsorbed proteins can be regulated by the HAp content. In summary, a tunable protein reservoir was formed on a porous polyester membrane.
采用改进的交替浸泡工艺（ASP）研究了多孔聚酯膜上的生物矿化作用。结果表明，离子迁移对羟基磷灰石（HAp）形成的影响至关重要。通过将乙醇混入水溶液中来降低表面张力，从而改善离子迁移。对所得的杂化材料进行了钙含量评估；利用扫描电子显微镜（SEM）、X射线衍射（XRD）和红外光谱（IR）对其结构进行了分析；并研究了蛋白质吸附情况。形成的HAp量由ASP循环次数以及混合溶剂中的乙醇含量控制。随着HAp形成量的增加，可通过SEM、IR和XRD对形成的结构进行验证。使用白蛋白、γ-球蛋白和纤维蛋白原研究了蛋白质吸附情况，吸附的蛋白质量与形成的HAp量具有良好的相关性。该结果表明，吸附蛋白的总量可由HAp含量调节。总之，在多孔聚酯膜上形成了一个可调谐的蛋白质储存库。
