Madhumathi K, Binulal N S, Nagahama H, Tamura H, Shalumon K T, Selvamurugan N, Nair S V, Jayakumar R
Amrita Centre for Nanosciences, Amrita Institute of Medical Sciences and Research Centre, Amrita Viswa Vidyapeetham University, Kochi 682026, India.
Int J Biol Macromol. 2009 Jan 1;44(1):1-5. doi: 10.1016/j.ijbiomac.2008.09.013. Epub 2008 Oct 1.
Beta-chitin is a biopolymer principally found in shells of squid pen. It has the properties of biodegradability, biocompatibility, chemical inertness, wound healing, antibacterial and anti-inflammatory activities. Hydroxyapatite (HAp) is a natural inorganic component of bone and teeth and has osteoconductive property. In this work, beta-chitin-HAp composite membranes were prepared by alternate soaking of beta-chitin membranes in CaCl2 (pH 7.4) and Na2HPO4 solutions for 2 h in each solution. After 1, 3 and 5 cycles of immersion, beta-chitin membranes were characterized using the SEM, FT-IR, EDS and XRD analyses. The results showed the presence of apatite layer on surface of beta-chitin membranes, and the amounts of size and deposition of apatite layers were increased with increasing number of immersion cycles. Human mesenchymal stem cells (hMSCs) were used for evaluation of the biocompatibility of pristine as well as composite membranes for tissue engineering applications. The presence of apatite layers on the surface of beta-chitin membranes increased the cell attachment and spreading suggesting that beta-chitin-HAp composite membranes can be used for tissue engineering applications.
β-几丁质是一种主要存在于鱿鱼笔壳中的生物聚合物。它具有生物可降解性、生物相容性、化学惰性、伤口愈合、抗菌和抗炎活性。羟基磷灰石（HAp）是骨骼和牙齿的天然无机成分，具有骨传导性。在这项工作中，通过将β-几丁质膜交替浸泡在CaCl2（pH 7.4）和Na2HPO4溶液中，每种溶液浸泡2小时，制备了β-几丁质-HAp复合膜。经过1、3和5个浸泡循环后，使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、能谱分析（EDS）和X射线衍射（XRD）分析对β-几丁质膜进行表征。结果表明，β-几丁质膜表面存在磷灰石层，并且磷灰石层的尺寸和沉积量随着浸泡循环次数的增加而增加。人骨髓间充质干细胞（hMSCs）用于评估原始膜和复合膜在组织工程应用中的生物相容性。β-几丁质膜表面磷灰石层的存在增加了细胞附着和铺展，这表明β-几丁质-HAp复合膜可用于组织工程应用。
