Tang Tao, Zheng Jian-Wei, Chen Bo, Li Hong, Li Xi, Xue Ke-Ying, Ai Xing, Zou Sheng-Quan
Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hepatobiliary Pancreat Dis Int. 2007 Jun;6(3):303-7.
Targeting is a new therapeutic tool for malignant tumor as a result of combining nanotechnology with chemotherapeutics. The aim of our study was to investigate the effects of magnetic nanoparticles enveloping a chemotherapeutic drug on human cholangiocarcinoma xenografts in nude mice.
The human cholangiocarcinoma xenograft model was established in nude mice with the QBC939 cell line. The nude mice were randomly assigned to 7 groups. 0.9% saline or magnetic nanoparticles, including high (group 2), medium (group 4) and low (group 5) dosages, were given to nude mice through the tail vein 20 days after the QBC939 cell line was implanted. Calculations were made 35 days after treatment in order to compare the volumes, inhibition ratios and growth curves of the tumors in each group. Mice in each group were sacrificed randomly to collect tumor tissues and other organs for electron microscopy and pathological examination.
The high and medium dosage groups were significantly different from the control group (P<0.05). The tumor inhibition ratios for the high, medium and low dosage groups were 39.6%, 14.6% and 7.9%, respectively. The tumor growth curve of groups 5, 4, and 2 changed slowly in turn. The high and medium groups showed cell apoptosis under an electron microscope.
Magnetic nanoparticles can inhibit the growth of human cholangiocarcinoma xenografts in nude mice.
靶向治疗作为纳米技术与化疗药物相结合的一种新型恶性肿瘤治疗手段。本研究旨在探讨包裹化疗药物的磁性纳米颗粒对裸鼠人胆管癌移植瘤的影响。
采用QBC939细胞系在裸鼠体内建立人胆管癌移植瘤模型。将裸鼠随机分为7组。在接种QBC939细胞系20天后，通过尾静脉向裸鼠注射0.9%生理盐水或高（第2组）、中（第4组）、低（第5组）剂量的磁性纳米颗粒。治疗35天后进行计算，以比较各组肿瘤的体积、抑制率和生长曲线。随机处死每组小鼠，收集肿瘤组织和其他器官进行电子显微镜检查和病理检查。
高剂量组和中剂量组与对照组差异有统计学意义（P<0.05）。高、中、低剂量组的肿瘤抑制率分别为39.6%、14.6%和7.9%。第5、4、2组的肿瘤生长曲线依次缓慢变化。高剂量组和中剂量组在电子显微镜下可见细胞凋亡。
磁性纳米颗粒可抑制裸鼠人胆管癌移植瘤的生长。
