Sun Jia-Yan, Tu Jin-Dong, Fan Shu-Wei, Wu Jian-Guo, Shi Chun-Hai
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Yi Chuan. 2007 Apr;29(4):475-82. doi: 10.1360/yc-007-0475.
The mature seeds of rapeseed (Brassica napus L.) from variety Gaoyou 605 were treated with g-rays and Ethyl Methan Sulfonate (EMS). 152 mutants (12.67% of M2 population) with mutative traits, including the mutation of leaf color, leaf shape, plant height, number and angle of branches, diameter of main stalk, color of stalk and flower, number and size of petals, pistil shape, male sterility, bud death and date of bloom were found in screened M2 progenies, which have been identified in M3. The mutants of cotyledon and root traits were also screened by hydroponics culture and their total mutant frequency were estimated at 12.78% and 7.07% in M3, respectively. Identification of M4 showed that these mutations could be inherited stably. The mutant library including the mutants of leaf, plant-type, flower, cotyledon, root and physiological traits had been built in present experiment. These mutants might be used as important germplasm for rapeseed breeding and functional genomics study.
用γ射线和甲基磺酸乙酯（EMS）处理甘蓝型油菜（Brassica napus L.）品种高邮605的成熟种子。在筛选的M2后代中发现了152个具有突变性状的突变体（占M2群体的12.67%），包括叶色、叶形、株高、分枝数和分枝角度、主茎直径、茎和花的颜色、花瓣数和大小、雌蕊形状、雄性不育、芽死亡和开花日期的突变，这些突变体已在M3中得到鉴定。通过水培法筛选了子叶和根性状的突变体，在M3中其总突变频率分别估计为12.78%和7.07%。M4的鉴定表明这些突变可以稳定遗传。本实验构建了包括叶、株型、花、子叶、根和生理性状突变体的突变体库。这些突变体可作为油菜育种和功能基因组学研究的重要种质资源。
