Zhai Guang-Hua, Wen Ping, Guo Lan-Fang, Chen Lu
School of Medical Technology, Jiangsu University, Zhenjiang 212013, China.
Yi Chuan. 2007 May;29(5):541-6. doi: 10.1360/yc-007-0541.
The purpose of this study was to explore the frequency of apolipoprotein A5 (APOA5) -1131T/C polymorphism in Zhenjiang and its effects on lipid metabolism and insulin resistance in type II diabetes mellitus(DM) patients. The genotypes of APOA5 -1131T/C polymorphism were determined in 152 healthy individuals and 71 type II DM patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum levels of lipids, glucose and insulin in these subjects were also estimated by biochemical methods. The frequency of the APOA5-1131C allele in DM patients was significantly higher than that of the control group (0.430 vs 0.296, P = 0.006). When compared with the TT genotype, CC homozygotes had a significantly increased DM risk (OR=3.75, 95% CI: 1.57-8.92). In the DM group, the serum levels of triglyceride (TG) of C carriers (TC+CC) were significantly higher than those of non-C carriers (TT) (P 0.01), and serum levels of total cholesterol (TC) and low density lipoprotein cholesterol(LDL-c) in subjects with the CC genotype were also significantly higher than those with the TT genotype (P 0.05). However, there was no significance in profiles of insulin resistance in various genotypes in both groups. The APOA5 single nucleotide polymorphism was associ-ated with serum TG level in the population. The -1131C allele contributed to the increase of serum levels of TG, TC and LDL-c and but had no effect on profiles of insulin resistance in DM patients. The APOA5 -1131C allele may be associated with increased susceptibility to type II diabetes mellitus.
本研究旨在探讨镇江地区载脂蛋白A5（APOA5）-1131T/C基因多态性的频率及其对2型糖尿病（DM）患者脂质代谢和胰岛素抵抗的影响。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对152例健康个体和71例2型DM患者进行APOA5 -1131T/C基因多态性的基因型检测。同时采用生化方法检测这些受试者的血脂、血糖和胰岛素水平。DM患者中APOA5-1131C等位基因频率显著高于对照组（0.430比0.296，P = 0.006）。与TT基因型相比，CC纯合子患DM的风险显著增加（OR=3.75，95%CI：1.57-8.92）。在DM组中，C等位基因携带者（TC+CC）的血清甘油三酯（TG）水平显著高于非C等位基因携带者（TT）（P<0.01），CC基因型受试者的血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-c）水平也显著高于TT基因型受试者（P<0.05）。然而，两组不同基因型的胰岛素抵抗情况无显著差异。APOA5单核苷酸多态性与人群血清TG水平相关。-1131C等位基因导致DM患者血清TG、TC和LDL-c水平升高，但对胰岛素抵抗情况无影响。APOA5 -1131C等位基因可能与2型糖尿病易感性增加有关。
