Yan Sheng-Kai, Cheng Xin-Qi, Song Yao-Hong, Xiao Xin-Hua, Bi Nan, Chen Bao-Sheng
Division of Clinical Biochemistry, Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Clin Chem Lab Med. 2005;43(6):607-12. doi: 10.1515/CCLM.2005.105.
Type 2 diabetes mellitus (DM) is associated with significant abnormalities of lipoprotein metabolism and coronary heart disease (CHD). The most commonly recognized lipid abnormality in type 2 DM is hypertriglyceridemia, which is known to be an independent risk factor for CHD in diabetics. The -1131T-->C polymorphism found in the newly identified apolipoprotein A5 ( APOA5 ) gene has been found to be associated with elevated plasma triglyceride (TG) concentrations in different racial groups. In this study, DNA samples from 155 control subjects, 172 type 2 diabetics and 113 type 2 DM patients with CHD were analyzed to examine the influence of APOA5 1131T-->C polymorphism on plasma lipids and the susceptibility to CHD in type 2 diabetics. The frequency of the APOA5 -1131C allele in the DM+CHD group was significantly higher than that of control subjects (37.2% vs. 27.7%, p=0.021). The distribution of the APOA5 -1131T-->C genotypes (TT, TC and CC) was 36.3%, 53.1% and 10.6% in type 2 DM patients with CHD, and 53.6%, 37.4% and 9.0% in controls, respectively (p=0.018). The frequencies of alleles and genotypes in type 2 diabetics were not significant compared to controls. In controls, plasma TG concentrations in subjects with the TT genotype were significantly lower than in those with TC/CC (0.92, 1.28 and 1.35 mmol/L for TT, TC and CC, respectively; p = 0.003 by ANOVA). These data suggest that the APOA5 -1131T-->C polymorphism might play a role in elevated plasma TG levels in type 2 diabetic patients in the Chinese population.
2型糖尿病（DM）与脂蛋白代谢显著异常及冠心病（CHD）相关。2型糖尿病中最常见的脂质异常是高甘油三酯血症，已知其为糖尿病患者发生冠心病的独立危险因素。新发现的载脂蛋白A5（APOA5）基因中的 -1131T→C多态性已被发现与不同种族人群血浆甘油三酯（TG）浓度升高有关。在本研究中，分析了155名对照受试者、172名2型糖尿病患者和113名患有冠心病的2型糖尿病患者的DNA样本，以研究APOA5 1131T→C多态性对2型糖尿病患者血脂及冠心病易感性的影响。糖尿病合并冠心病组中APOA5 -1131C等位基因频率显著高于对照受试者（37.2%对27.7%，p = 0.021）。2型糖尿病合并冠心病患者中APOA5 -1131T→C基因型（TT、TC和CC）的分布分别为36.3%、53.1%和10.6%，对照组分别为53.6%、37.4%和9.0%（p = 0.018）。与对照组相比，2型糖尿病患者的等位基因和基因型频率无显著差异。在对照组中，TT基因型受试者的血浆TG浓度显著低于TC/CC基因型受试者（TT、TC和CC基因型分别为0.92、1.28和1.35 mmol/L；方差分析p = 0.003）。这些数据表明，APOA5 -1131T→C多态性可能在中国人群2型糖尿病患者血浆TG水平升高中起作用。
