Xue Ke, Li Feng, Luo Guang-Bin, Huang Wei-Wei, Chen Xue-Jin
College of Animal Science and Vet Medicine of Shenyang Agricultural University, Shenyang 110161, China.
Yi Chuan. 2007 May;29(5):570-4. doi: 10.1360/yc-007-0570.
It has been reported that homologous recombination with Red system has been successfully used for knock-out. We try to work on the construction of the expression vector of Mammary Gland with Red system. This study takes CSN2 as a vector for gene target, which contains the complete bovine beta casein gene. Different homologous arms were designed and the CDS region of the beta casein gene was successfully knocked out. The efficiency was also explored for knocking out different DNA fragment. Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region.
