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Minimum length of homology arms required for effective Red/ET recombination.

作者信息

Fujimoto Rika, Osakabe Toshihiko, Saito Masumichi, Kurosawa Nobuyuki, Isobe Masaharu

机构信息

Graduate School of Innovative Life Science, University of Toyama, Japan.

出版信息

Biosci Biotechnol Biochem. 2009 Dec;73(12):2783-6. doi: 10.1271/bbb.90584. Epub 2009 Dec 7.

DOI:10.1271/bbb.90584
PMID:19966478
Abstract

The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.

摘要

相似文献

1
Minimum length of homology arms required for effective Red/ET recombination.
Biosci Biotechnol Biochem. 2009 Dec;73(12):2783-6. doi: 10.1271/bbb.90584. Epub 2009 Dec 7.
2
Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast.通过酵母中的同源重组进行体内克隆所需的序列同源性的最小长度。
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3
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.利用体外同源重组通过SLIC技术生成重组DNA。
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4
[Determination of the minimal length DNA homologous region required for plasmid integration into the Bacillus subtilis chromosome via homologous recombination].[通过同源重组将质粒整合到枯草芽孢杆菌染色体所需的最小长度DNA同源区域的测定]
Genetika. 1992 Jul;28(7):38-45.
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Site-directed mutagenesis of multi-copy-number plasmids: Red/ET recombination and unique restriction site elimination.多拷贝数质粒的定点诱变：Red/ET重组与独特限制酶切位点消除
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Analysis of shuffled gene libraries.随机排列基因文库分析
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Random strand transfer recombination (RSTR) for homology-independent nucleic acid recombination.用于非同源核酸重组的随机链转移重组（RSTR）
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靶向选择性共聚合酶链反应：一种从单个细胞高通量生产重组单克隆抗体的稳健、快速方法。
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