Mäkelä M, Söderling E, Paunio K, Talonpoika J, Hyyppä T
Institute of Dentistry, University of Turku, Finland.
Scand J Dent Res. 1991 Oct;99(5):413-23. doi: 10.1111/j.1600-0722.1991.tb01049.x.
Crevicular fluid (CF) analysis is a potential tool for site-specific diagnosis of periodontal disease activity. In this study, CF was collected using a novel washing method from 91 sites in 18 adult periodontitis patients both before and after conventional periodontal treatment. The sites studied were classified according to their clinical status and the number of polymorphonuclear leukocytes (PMN's) in CF samples. CF proteins were analyzed from individual sites with gel electrophoresis (SDS-PAGE). Furthermore, both the cell-bound and soluble neutral proteolytic activities of the samples were determined. Albumin was the main protein both in healthy and slightly inflamed sites. The most severely inflamed sites were characterized by high levels of low molecular weight (LMW) proteins (mol. weight ca. 12,000) and strong cell-bound neutral proteolytic activity. Scaling and root planing reduced both the LMW proteins and neutral proteolytic activity markedly in pockets responding well to treatment. The levels of the LMW proteins in CF correlated with the cell-bound neutral proteolytic activity, which reflected the number of PMN's in the sample. The present results suggest that the appearance of the LMW proteins in CF is associated with the periodontal inflammatory status of the site.
龈沟液（CF）分析是一种用于牙周疾病活动位点特异性诊断的潜在工具。在本研究中，采用一种新型冲洗方法，在常规牙周治疗前后，从18例成年牙周炎患者的91个位点采集龈沟液。所研究的位点根据其临床状态以及龈沟液样本中多形核白细胞（PMN）的数量进行分类。通过凝胶电泳（SDS-PAGE）对各个位点的龈沟液蛋白质进行分析。此外，还测定了样本的细胞结合型和可溶性中性蛋白水解活性。在健康和轻度炎症位点，白蛋白是主要蛋白质。炎症最严重的位点其特征是低分子量（LMW）蛋白质（分子量约12,000）水平较高且细胞结合型中性蛋白水解活性较强。在对治疗反应良好的牙周袋中，龈上洁治和根面平整显著降低了低分子量蛋白质和中性蛋白水解活性。龈沟液中低分子量蛋白质的水平与细胞结合型中性蛋白水解活性相关，后者反映了样本中PMN的数量。目前的结果表明，龈沟液中低分子量蛋白质的出现与该位点的牙周炎症状态有关。
