Mukherjee S, Novack N, Blaustein D, Chambers D A
J Clin Periodontol. 1987 Jul;14(6):320-5. doi: 10.1111/j.1600-051x.1987.tb00978.x.
The ligature-induced periodontitis model was used in beagle dogs to compare and contrast profiles of crevicular fluid (CF) proteins collected from gingivitis and periodontitis sites. The protein profiles of CF and serum were determined by 2-dimensional gel electrophoresis (2-D PAGE) using a silver stain. 2-D PAGE showed that CF contained proteins with molecular weight 16 K or less and many proteins with molecular weights between 64 K and 16 K in the isoelectric pH between approximately 5.8 and 6.8. The number of such proteins was greater in samples collected from the ligated (periodontitis) side compared to the non-ligated (gingivitis) side. Thus, analysis by 2-D PAGE revealed differences between CF samples from gingivitis and ligature-induced periodontitis sites. This study suggests that analysis of human CF by 2-D PAGE may be useful in diagnosis and investigation of the pathogenesis of periodontitis.
在比格犬中使用结扎诱导的牙周炎模型，以比较和对比从牙龈炎和牙周炎部位收集的龈沟液（CF）蛋白质谱。使用银染通过二维凝胶电泳（2-D PAGE）测定CF和血清的蛋白质谱。二维凝胶电泳显示，CF含有分子量为16K或更低的蛋白质，以及许多分子量在64K和16K之间、等电点pH约为5.8至6.8的蛋白质。与未结扎（牙龈炎）侧相比，从结扎（牙周炎）侧收集的样本中此类蛋白质的数量更多。因此，二维凝胶电泳分析揭示了来自牙龈炎和结扎诱导的牙周炎部位的CF样本之间的差异。这项研究表明，通过二维凝胶电泳分析人CF可能有助于牙周炎的诊断和发病机制研究。
