Shimakage Misuzu, Kawahara Kunimitsu, Harada Shizuko, Sasagawa Toshiyuki, Shinka Toshiaki, Oka Toshitsugu
Clinical Reseach Institute, National Hospital Organization, Osaka National Hospital, Osaka 540-0006, Japan.
Oncol Rep. 2007 Jul;18(1):41-6.
There have been few studies regarding the etiology of renal cell carcinoma. To examine the possible involvement of Epstein-Barr virus (EBV) in this disease, 9 renal cell carcinoma (RCC), 2 nephroblastoma (Wilms' tumor) and 2 RCC cell lines were subjected to mRNA in situ hybridization and indirect immunofluorescence staining. Messenger RNA in situ hybridization using BamHIW, EBNA LP, EBNA 2 and EBER1 probes of EBV revealed signals in all the examined samples, although some samples showed weak signals using the EBNA LP probe. Indirect immunofluorescence staining using anti-EBNA LP, anti-EBNA2, anti-LMP1 and anti-BZLF1 antibodies showed definitive fluorescence. PCR also revealed EBV DNA in all 8 RCC specimens including 7 cases other than hybridization and fluorescence. EBV infected all the RCC and nephroblastoma irrespective of the histological or clinical stage. On the other hand, EBV expression was stronger in papillary and clear cell-type RCC than chromophobe cell-type, as well as being stronger in the higher grades of RCC. These results suggest that the expression of EBV may be involved in the pathogenesis of RCC and nephroblastoma.
关于肾细胞癌的病因学研究较少。为了研究爱泼斯坦-巴尔病毒（EBV）在该疾病中可能的作用，对9例肾细胞癌（RCC）、2例肾母细胞瘤（威尔姆斯瘤）和2种RCC细胞系进行了mRNA原位杂交和间接免疫荧光染色。使用EBV的BamHIW、EBNA LP、EBNA 2和EBER1探针进行mRNA原位杂交，在所有检测样本中均发现信号，不过有些样本使用EBNA LP探针时信号较弱。使用抗EBNA LP、抗EBNA2、抗LMP1和抗BZLF1抗体进行间接免疫荧光染色显示出明确的荧光。PCR也在所有8例RCC标本中检测到EBV DNA，其中包括7例除杂交和荧光检测外的病例。无论组织学或临床分期如何，EBV均感染了所有RCC和肾母细胞瘤。另一方面，EBV在乳头状和透明细胞型RCC中的表达强于嫌色细胞型，并且在高分级RCC中表达更强。这些结果表明，EBV的表达可能参与了RCC和肾母细胞瘤的发病机制。
