Sugawara Y, Mizugaki Y, Uchida T, Torii T, Imai S, Makuuchi M, Takada K
Cancer Institute, Hokkaido University School of Medicine, N15 W7, Kita-ku, Sapporo, 060-8638, Japan.
Virology. 1999 Apr 10;256(2):196-202. doi: 10.1006/viro.1999.9619.
In this study, we investigated the presence of Epstein-Barr virus (EBV) in liver tissue from 35 patients with hepatocellular carcinoma (HCC). EBV DNA was detected in 13 patients (37%) by Southern blot hybridization. In 10 of these patients, EBV DNA was present in tumor tissue only, whereas in the other 3, it was detected in both tumor and nontumor tissues. The quantity of EBV DNA detected was equivalent to 1-10 viral DNA molecules/100 cells. EBV-determined nuclear antigen was detected in 7-13% of the carcinoma cells in three tumor tissue samples that contained approximately one copy of the EBV genome/10 cells. A single terminal fragment of EBV DNA was identified in these tissues, suggesting that the EBV-infected cells in HCC represent clonal proliferation. Western blotting and reverse transcription-polymerase chain reaction analyses demonstrated that these three tumor tissue specimens were positive for EBV-determined nuclear antigen 1 and BamHI A transcripts but were negative for the other latent EBV products, including EBV-encoded small RNA. The results indicated that there is a high EBV load in HCC tissue and that all of the HCC tissue examined showed a novel pattern of EBV latency characterized by absence of EBV-encoded small RNA expression.
在本研究中，我们调查了35例肝细胞癌（HCC）患者肝组织中EB病毒（EBV）的存在情况。通过Southern印迹杂交在13例患者（37%）中检测到EBV DNA。在这些患者中，10例患者的EBV DNA仅存在于肿瘤组织中，而在另外3例患者中，肿瘤组织和非肿瘤组织中均检测到EBV DNA。检测到的EBV DNA数量相当于1 - 10个病毒DNA分子/100个细胞。在三个肿瘤组织样本中，约10个细胞含有一个EBV基因组拷贝，7% - 13%的癌细胞中检测到EBV核抗原。在这些组织中鉴定出EBV DNA的单个末端片段，表明HCC中受EBV感染的细胞代表克隆增殖。蛋白质免疫印迹和逆转录 - 聚合酶链反应分析表明，这三个肿瘤组织标本中EBV核抗原1和BamHI A转录本呈阳性，但其他潜伏性EBV产物，包括EBV编码的小RNA呈阴性。结果表明，HCC组织中EBV载量较高，并且所有检测的HCC组织均显示出一种以缺乏EBV编码的小RNA表达为特征的新型EBV潜伏模式。
