Shi Xiaoyan, Chen Gang, Xing Hui, Weng Danhui, Bai Xiangyang, Ma Ding
Cancer Biology Research Center, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Hubei 430030, P.R. China.
Oncol Rep. 2007 Jul;18(1):241-7.
The study was conducted to clarify the expression and biological significance of VEGF-C and VEGFR-3 in human cervical cancer cell lines and to investigate the correlation between VEGF-C and cyclooxygenase-2 (COX-2) expression in these cells. Flow cytometry, Western blotting, RT-PCR, cell immunochemistry assay, cell proliferation assay, in vitro invasion assay and cell immunochemistry assay were used to detect the gene expression and to evaluate the biological features in test cell lines. VEGF-C expression was low while VEGFR-3 was quiet high in all cell lines detected. COX-2 expression coincided with that of VEGF-C. Recombinant human VEFG-C-treated HeLa cells showed increased proliferation and invasion in a dose-depended manner while NS398, a specific inhibitor of COX-2, blocked the invasive ability of HeLa. VEGF-C and its VEGFR-3 played a crucial role in the regulation of tumor growth and metastasis in cervical cell lines, and COX-2 might be a regulator of VEGF-C expression.
本研究旨在阐明VEGF-C和VEGFR-3在人宫颈癌细胞系中的表达及生物学意义，并探讨这些细胞中VEGF-C与环氧合酶-2（COX-2）表达之间的相关性。采用流式细胞术、蛋白质印迹法、逆转录-聚合酶链反应、细胞免疫化学分析、细胞增殖分析、体外侵袭分析和细胞免疫化学分析来检测基因表达，并评估受试细胞系的生物学特性。在所有检测的细胞系中，VEGF-C表达较低，而VEGFR-3表达较高。COX-2表达与VEGF-C表达一致。重组人VEFG-C处理的HeLa细胞显示出增殖和侵袭增加，且呈剂量依赖性，而COX-2的特异性抑制剂NS398可阻断HeLa的侵袭能力。VEGF-C及其VEGFR-3在宫颈癌细胞系的肿瘤生长和转移调节中起关键作用，COX-2可能是VEGF-C表达的调节因子。
