Franc Magdalena, Kachel-Flis Agata, Michalski Bogdan, Fila-Daniłow Anna, Mazurek Urszula, Michalski Mateusz, Michalska Anna, Kuczerawy Ilona, Skrzypulec-Plinta Violetta
Medical Department, Medical University of Silesia in Katowice, Poland.
Department of Oncology, St. Leszczynski Memorial Hospital in Katowice, Poland.
Prz Menopauzalny. 2015 Jun;14(2):112-7. doi: 10.5114/pm.2015.49397. Epub 2015 Jun 22.
The aim of the present study was to evaluate the profile of VEGF-C gene expression in particular stages of cervical cancer (IB-IIIB) and to estimate the correlation between VEGF-C mRNA quantity profile and clinical stage.
Material for molecular analysis consisted of cervical cancer tissue specimens collected from 38 women (10, 15, 13 cases were classified as IB, IIB and IIIB, respectively). The control group was composed of normal cervical tissues collected from 10 women who underwent hysterectomy for non-oncological reasons. The number of VEGF-C mRNA copies in particular groups was estimated by the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method.
In the control group the average number of mRNA copies was 134 ± 36 (median: 106), in a group with stage IB it was 16 077 ± 7090 (median: 580), for stage IIB - 35 019 ± 8945 (median: 40 870). The highest number of mRNA VEGF-C copies was derived in a group of patients with cervical cancer of stage IIIB. The average quantity was 56 155 ± 12 470, whereas median 55 981. A statistically significantly higher level of VEGF-C gene expression was disclosed in cervical cancer specimens with stage IIB and IIIB than in the control group. In stage IIIB, the VEGF-C gene expression was significantly higher than in specimens derived from individuals in stage IB.
In squamous cell carcinoma of the uterine cervix of stage IB-IIIB genes involved in lymphangiogenesis, especially VEGF-C, are expressed, which expression increases as the clinical stage of cervical cancer is higher.
本研究旨在评估血管内皮生长因子C（VEGF-C）基因在宫颈癌特定阶段（IB-IIIB期）的表达情况，并估计VEGF-C mRNA数量与临床分期之间的相关性。
分子分析材料包括从38名女性收集的宫颈癌组织标本（分别有10例、15例和13例被分类为IB期、IIB期和IIIB期）。对照组由10名因非肿瘤原因接受子宫切除术的女性的正常宫颈组织组成。通过逆转录定量聚合酶链反应（RT-qPCR）方法估计特定组中VEGF-C mRNA拷贝数。
对照组中mRNA拷贝数的平均值为134±36（中位数：106），IB期组为16077±7090（中位数：580），IIB期组为35019±8945（中位数：40870）。IIIB期宫颈癌患者组中VEGF-C mRNA拷贝数最高。平均数量为56155±12470，而中位数为55981。IIB期和IIIB期宫颈癌标本中VEGF-C基因表达水平在统计学上显著高于对照组。在IIIB期，VEGF-C基因表达显著高于IB期个体的标本。
在IB-IIIB期子宫颈鳞状细胞癌中，参与淋巴管生成的基因，尤其是VEGF-C，有表达，且随着宫颈癌临床分期升高其表达增加。
