Brys Magdalena, Semczuk Andrzej, Rechberger Tomasz, Krajewska Wanda M
Department of Cytobiochemistry, University of Lodz, Lodz 90-237, Poland.
Oncol Rep. 2007 Jul;18(1):261-5.
Overexpression of the erbB-1 (EGFR, epidermal growth factor receptor) and erbB-2 (HER2/neu) proteins contributes to the aggressive behavior of malignant tumors originating from the endometrium. We currently examined whether the trend of these proteins to overexpression is a direct effect of their gene transcriptional activities. Expression of the erbB-1/erbB-2 genes was measured applying the quantitative RT-PCR technique in 25 uterine carcinomas, 12 normal endometria, a carcinosarcoma and a case of botryoid sarcoma of the uterine cervix. We showed that erbB-1 mRNA was overexpressed in 48% (12/25) and erbB-2 mRNA was overexpressed in 8% (2/25) of the analysed tumors. The level of expression appeared to be significantly higher in the malignant tumors as compared to the benign ones for erbB-1 and for erbB-2 (p=0.0001 and p=0.008, respectively). A significant correlation between erbB-1 overexpression and tumor differentiation was found (Spearman rank correlation test, p<0.001). Concomitant erbB-1 and erbB-2 overexpression was detected only in 1 out of 25 (4%) uterine neoplasms. erbB-1 was overexpressed in a sarcoma botryoides of the uterine cervix. Our data suggest that erbB-1/erbB-2 overexpression is a direct effect of higher than normal transcriptional activity of the encoding genes in a subset of human endometrial carcinomas.
erbB-1（表皮生长因子受体，EGFR）和erbB-2（HER2/neu）蛋白的过表达促成了源自子宫内膜的恶性肿瘤的侵袭性行为。我们目前研究了这些蛋白过表达的趋势是否是其基因转录活性的直接效应。应用定量逆转录聚合酶链反应（RT-PCR）技术检测了25例子宫癌、12例正常子宫内膜、1例子宫癌肉瘤及1例子宫颈葡萄状肉瘤中erbB-1/erbB-2基因的表达。我们发现，在所分析的肿瘤中，48%（12/25）的肿瘤erbB-1信使核糖核酸（mRNA）过表达，8%（2/25）的肿瘤erbB-2 mRNA过表达。与良性肿瘤相比，erbB-1和erbB-2在恶性肿瘤中的表达水平明显更高（p分别为0.0001和0.008）。发现erbB-1过表达与肿瘤分化之间存在显著相关性（Spearman等级相关检验，p<0.001）。在25例子宫肿瘤中，仅1例（4%）检测到erbB-1和erbB-2同时过表达。erbB-1在1例子宫颈葡萄状肉瘤中过表达。我们的数据表明，erbB-1/erbB-2过表达是一部分人类子宫内膜癌中编码基因转录活性高于正常水平的直接效应。
