Zhou Yan, Bianco Katherine, Huang Ling, Nien Jyh K, McMaster Michael, Romero Roberto, Fisher Susan J
Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA, USA.
Cell Tissue Res. 2007 Sep;329(3):559-69. doi: 10.1007/s00441-007-0428-0. Epub 2007 Jun 5.
The maternal-fetal interface, a chimeric structure, is formed when fetal cytotrophoblasts (CTBs) from the placenta invade the uterine wall and its resident vasculature. In preeclampsia (PE), interstitial and endovascular invasion are often shallow, and fewer spiral arterioles are breached in toto. Our previous work has shown that faulty CTB differentiation to an invasive phenotype is a contributing factor. Here, we have tested the hypothesis that the constellation of morphological and molecular defects that are associated with PE are unique to this condition. Specifically, we have compared the histology of the maternal-fetal interface and CTB expression of stage-specific antigens in PE and in preterm labor (PTL) with or without inflammation. In the absence of inflammation, biopsies obtained after PTL were near normal at histological and molecular levels. In accord with previously published data, PE had severe negative effects on the endpoints analyzed. Biopsies obtained after PTL with inflammation had an intermediate phenotype. Our results suggest that the maternal-fetal interface from cases of PTL without inflammation can be used for comparative purposes, e.g., as age-matched controls, in studies of the effects of PE on cells in this region.
母胎界面是一种嵌合结构，当胎盘的胎儿细胞滋养层（CTB）侵入子宫壁及其固有脉管系统时形成。在子痫前期（PE）中，间质和血管内侵入通常较浅，完全被穿透的螺旋小动脉较少。我们之前的研究表明，CTB向侵袭性表型的分化异常是一个促成因素。在此，我们检验了一个假设，即与PE相关的形态学和分子缺陷组合是这种病症所特有的。具体而言，我们比较了PE以及有或无炎症的早产（PTL）中母胎界面的组织学和CTB阶段特异性抗原的表达。在没有炎症的情况下，PTL后获取的活检组织在组织学和分子水平上接近正常。与先前发表的数据一致，PE对所分析的终点有严重负面影响。有炎症的PTL后获取的活检组织具有中间表型。我们的结果表明，无炎症的PTL病例的母胎界面可用于比较目的，例如，作为年龄匹配的对照，用于研究PE对该区域细胞的影响。
