Mahidol Chulabhorn, Na-Nakorn Uthairat, Sukmanomon Srijanya, Taniguchi Nobuhiko, Nguyen Thuy T T
Chulabhorn Research Institute, Vibhavadee-Rangsit Highway, Bangkok, 10210, Thailand.
Mar Biotechnol (NY). 2007 May-Jun;9(3):352-9. doi: 10.1007/s10126-006-6137-y. Epub 2007 Feb 15.
Sequence variation of the mitochondrial DNA 16S rRNA region of the Asian moon scallop, Amusium pleuronectes, was surveyed in seven populations along the coast of Thailand. A total of 16 unique haplotypes were detected among 174 individuals with a total 27 variable sites out of 534 bp sequenced. The mitochondrial haplotypes grouped into two distinct arrays (estimated to differ by about 2.62% to 2.99% nucleotide divergence) that characterized samples collected from the Gulf of Thailand versus the Andaman Sea. Low levels of intrapopulation variation were observed, while in contrast, significant divergence was observed between populations from the Gulf of Thailand and Andaman Sea. Results of AMOVA reveal a high F (ST) value (0.765) and showed that the majority of the total genetic variance (76.03%) occurred among groups (i.e., Andaman Sea and the Gulf of Thailand) and little among populations within the group (0.52%) and within populations (23.45%). The genetic differentiation between the populations recorded in the present study is similar to that observed in a variety of marine species in the Indo-Pacific. The implications of the findings for management of A. pleuronectes genetic resources in Thailand are discussed.
对泰国沿海七个种群的亚洲日月贝（Amusium pleuronectes）线粒体DNA 16S rRNA区域的序列变异进行了调查。在174个个体中总共检测到16种独特的单倍型，在测序的534 bp中共有27个可变位点。线粒体单倍型分为两个不同的阵列（估计核苷酸差异约为2.62%至2.99%），这两个阵列分别表征了从泰国湾和安达曼海采集的样本。观察到种群内变异水平较低，而相比之下，泰国湾和安达曼海的种群之间存在显著差异。分子方差分析（AMOVA）结果显示F（ST）值较高（0.765），表明总遗传变异的大部分（76.03%）发生在组间（即安达曼海和泰国湾），组内种群间变异较小（0.52%），种群内变异为23.45%。本研究记录的种群间遗传分化与在印度-太平洋地区各种海洋物种中观察到的情况相似。讨论了这些发现对泰国日月贝遗传资源管理的意义。
