Khamnamtong Bavornlak, Klinbunga Sirawut, Menasveta Piamsak
Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Klong Luang, Pathumthani, 12120, Thailand.
Biochem Genet. 2009 Feb;47(1-2):42-55. doi: 10.1007/s10528-008-9205-3. Epub 2008 Dec 12.
Genetic diversity and geographic differentiation of the giant tiger shrimp, Penaeus monodon, in Thai waters (Satun, Trang, Phangnga, and Ranong in the Andaman Sea and Chumphon and Trat in the Gulf of Thailand) were examined by COI polymorphism (N = 128). We observed 28 COI mitotypes across all investigated individuals. The sequence divergence between pairs of mitotypes was 0.00-20.76%. A neighbor-joining tree clearly indicated lineage separation of Thai P. monodon and large nucleotide divergence between interlineage mitotypes but limited divergence between intralineage mitotypes. High genetic diversity was found (mean sequence divergence = 6.604%, haplotype diversity = 0.716-0.927, pi = 2.936-8.532%). F-statistics (F(ST)) and an analysis of molecular variance (AMOVA) indicated that the gene pool of Thai P. monodon was not homogeneous but genetically differentiated intraspecifically (P < 0.05). Six samples of P. monodon could be allocated into three different genetic populations: Trat (A), Chumphon (B), and the Andaman samples Satun, Trang, Phangnga, and Ranong (C). Contradictory results regarding patterns of geographic differentiation previously reported by various molecular approaches were clarified by this study.
通过细胞色素氧化酶亚基I（COI）多态性（N = 128）对泰国海域（安达曼海的沙敦、董里、攀牙和拉廊以及泰国湾的春蓬和桐艾）的斑节对虾的遗传多样性和地理分化进行了研究。我们在所有调查个体中观察到28种COI单倍型。单倍型对之间的序列差异为0.00 - 20.76%。邻接法树清楚地表明了泰国斑节对虾的谱系分离以及谱系间单倍型之间的大核苷酸差异，但谱系内单倍型之间的差异有限。发现了高遗传多样性（平均序列差异 = 6.604%，单倍型多样性 = 0.716 - 0.927，π = 2.936 - 8.532%）。F统计量（F(ST)）和分子方差分析（AMOVA）表明，泰国斑节对虾的基因库不是同质的，而是在种内存在遗传分化（P < 0.05）。六个斑节对虾样本可分为三个不同的遗传群体：桐艾（A）、春蓬（B）以及安达曼海的样本沙敦、董里、攀牙和拉廊（C）。本研究澄清了先前各种分子方法所报告的关于地理分化模式的矛盾结果。
