Varela Miguel A, González-Tizón Ana, Mariñas Luis, Martínez-Lage Andrés
Departamento de Biología Celular y Molecular, Facultade de Ciencias, Universidade da Coruña, A Zapateira s/n, Coruña 15071, Spain.
Biochem Genet. 2007 Aug;45(7-8):565-78. doi: 10.1007/s10528-007-9097-7. Epub 2007 Jun 5.
The wide distribution of microsatellites in mussels of the Mytilus edulis complex (Mytilidae) enables the analysis of inter-simple-sequence repeat (ISSR) markers. The aim of this investigation was to assess genetic differentiation in six sampling localities distributed along the European Atlantic coast to expose the potential of these markers in genetic studies requiring the detection of low polymorphism and as a source of sequences for developing microsatellite markers. We detected low genetic structuring within each member of the Mytilus edulis complex. Nei and Li distances and AMOVA clustered the individuals studied into two groups. On the basis of these results two sampling localities coming from the M. edulis x M. galloprovincialis mosaic hybrid zone in Western Europe were assigned to one species. On the other hand, mussels of a sampling locality in the Baltic Sea were not significantly different from a pure M. edulis locality supporting an extensive introgression of M. edulis in these individuals. Finally, 148 microsatellites were found in the sequences of 51 ISSR markers, and two polymorphic microsatellite markers were developed.
微卫星在紫贻贝复合体（贻贝科）的贻贝中广泛分布，这使得对简单序列重复区间（ISSR）标记的分析成为可能。本研究的目的是评估沿欧洲大西洋海岸分布的六个采样地点的遗传分化情况，以揭示这些标记在需要检测低多态性的遗传研究中的潜力，并作为开发微卫星标记的序列来源。我们在紫贻贝复合体的每个成员内部检测到了低水平的遗传结构。内氏距离和李距离以及分子变异分析（AMOVA）将所研究的个体聚为两组。基于这些结果，来自西欧紫贻贝×加利福尼亚贻贝镶嵌杂交区的两个采样地点被归为一个物种。另一方面，波罗的海一个采样地点的贻贝与一个纯紫贻贝地点的贻贝没有显著差异，这支持了这些个体中紫贻贝的广泛渐渗。最后，在51个ISSR标记的序列中发现了148个微卫星，并开发了两个多态性微卫星标记。
