Xu T-J, Sun Y-N, Yuan Y-T, Liao Z, Wang R-X
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, P.R. China.
Genet Mol Res. 2010 Jul 13;9(3):1388-91. doi: 10.4238/vol9-3gmr856.
Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to seven, and the observed and expected heterozygosities ranged from 0.0333 to 0.8571 and from 0.3452 to 0.8267, respectively. Four loci deviated from Hardy-Weinberg equilibrium. These polymorphic microsatellite loci can be employed in genetic diversity analysis and molecular marker-assisted breeding of M. coruscus.
波纹巴非蛤是中国最重要的海水养殖贝类之一。利用波纹巴非蛤的一个表达序列标签文库和两个微卫星富集基因组文库，我们在一个测试群体中分离并鉴定了12个多态性微卫星；等位基因数从3到7不等，观察到的杂合度和期望杂合度分别从0.0333到0.8571和从0.3452到0.8267。四个位点偏离了哈迪-温伯格平衡。这些多态性微卫星位点可用于波纹巴非蛤的遗传多样性分析和分子标记辅助育种。
