Caspersen M B, Sørensen N M, Schrohl A S, Iversen P, Nielsen H J, Brünner N
Ciphergen Biosystems, Copenhagen, Denmark.
Int J Biol Markers. 2007 Apr-Jun;22(2):89-94. doi: 10.1177/172460080702200201.
Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and <0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.
结直肠癌（CRC）的早期检测可提高患者生存率。通过酶联免疫吸附测定（ELISA）检测血浆金属蛋白酶组织抑制剂1（TIMP-1）被认为是一种早期检测CRC的新方法。为了进一步研究血浆中TIMP-1的性质，采用了表面增强激光解吸/电离飞行时间质谱（SELDI TOF MS）。使用SELDI TOF MS和ELISA中的TIMP-1单克隆抗体对16名健康供体和14名CRC患者的血浆进行TIMP-1检测。应用TIMP-1抗体的SELDI TOF MS显示，人血浆TIMP-1的质量为25.1 kDa，并表现出几种异构体。与健康个体相比，两种方法均显示癌症患者的血浆TIMP-1值升高。ELISA组间分离的p值为0.0019，SELDI TOF MS的p值<0.0001。通过SELDI TOF MS评估，CRC并未从根本上影响TIMP-1的外观。
