• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用IMAC表面的SELDI-TOF MS全血清蛋白质组分析在特异性检测结直肠癌方面的局限性。

Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer.

作者信息

Wang Qi, Shen Jing, Li Zhen-fu, Jie Jian-zheng, Wang Wen-yue, Wang Jin, Zhang Zhong-tao, Li Zhi-xia, Yan Li, Gu Jin

机构信息

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Colorectal Surgery, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, PR China.

出版信息

BMC Cancer. 2009 Aug 19;9:287. doi: 10.1186/1471-2407-9-287.

DOI:10.1186/1471-2407-9-287
PMID:19689818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2743709/
Abstract

BACKGROUND

Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) analysis on serum samples was reported to be able to detect colorectal cancer (CRC) from normal or control patients. We carried out a validation study of a SELDI-TOF MS approach with IMAC surface sample processing to identify CRC.

METHODS

A retrospective cohort of 338 serum samples including 154 CRCs, 67 control cancers and 117 non-cancerous conditions was profiled using SELDI-TOF-MS.

RESULTS

No CRC "specific" classifier was found. However, a classifier consisting of two protein peaks separates cancer from non-cancerous conditions with high accuracy.

CONCLUSION

In this study, the SELDI-TOF-MS-based protein expression profiling approach did not perform to identify CRC. However, this technique is promising in distinguishing patients with cancer from a non-cancerous population; it may be useful for monitoring recurrence of CRC after treatment.

摘要

背景

据报道，对血清样本进行表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）分析能够从正常或对照患者中检测出结直肠癌（CRC）。我们开展了一项采用IMAC表面样本处理的SELDI-TOF MS方法来识别CRC的验证研究。

方法

使用SELDI-TOF-MS对一个包含154例CRC、67例对照癌症和117例非癌病症的338份血清样本的回顾性队列进行分析。

结果

未发现CRC“特异性”分类器。然而，一个由两个蛋白峰组成的分类器能以高精度将癌症与非癌病症区分开来。

结论

在本研究中，基于SELDI-TOF-MS的蛋白质表达谱分析方法未能识别出CRC。然而，这项技术在区分癌症患者与非癌人群方面很有前景；它可能有助于监测CRC治疗后的复发情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/61060e338c98/1471-2407-9-287-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/f4ab920e14e4/1471-2407-9-287-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/24c6b7f7d67c/1471-2407-9-287-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/915634ea1aab/1471-2407-9-287-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/61060e338c98/1471-2407-9-287-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/f4ab920e14e4/1471-2407-9-287-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/24c6b7f7d67c/1471-2407-9-287-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/915634ea1aab/1471-2407-9-287-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e41b/2743709/61060e338c98/1471-2407-9-287-4.jpg

相似文献

1
Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer.使用IMAC表面的SELDI-TOF MS全血清蛋白质组分析在特异性检测结直肠癌方面的局限性。
BMC Cancer. 2009 Aug 19;9:287. doi: 10.1186/1471-2407-9-287.
2
Preoperatively molecular staging with CM10 ProteinChip and SELDI-TOF-MS for colorectal cancer patients.术前使用CM10蛋白芯片和表面增强激光解吸电离飞行时间质谱对结直肠癌患者进行分子分期。
J Zhejiang Univ Sci B. 2006 Mar;7(3):235-40. doi: 10.1631/jzus.2006.B0235.
3
Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry.使用表面增强激光解吸电离飞行时间质谱法鉴定区分结直肠癌患者和健康对照的血清蛋白。
World J Gastroenterol. 2006 Mar 14;12(10):1536-44. doi: 10.3748/wjg.v12.i10.1536.
4
A serum proteomic pattern for the detection of colorectal adenocarcinoma using surface enhanced laser desorption and ionization mass spectrometry.一种使用表面增强激光解吸电离质谱法检测结肠直肠癌的血清蛋白质组模式。
Cancer Invest. 2006 Dec;24(8):747-53. doi: 10.1080/07357900601063873.
5
SELDI-TOF MS whole serum proteomic profiling with IMAC surface does not reliably detect prostate cancer.使用IMAC表面的SELDI-TOF MS全血清蛋白质组分析不能可靠地检测前列腺癌。
Clin Chem. 2008 Jan;54(1):53-60. doi: 10.1373/clinchem.2007.091496. Epub 2007 Nov 16.
6
SELDI-TOF serum proteomics and colorectal cancer: a current overview.SELDI-TOF 血清蛋白质组学与结直肠癌：当前概述。
Arch Physiol Biochem. 2010 Oct-Dec;116(4-5):188-96. doi: 10.3109/13813455.2010.495130. Epub 2010 Jul 8.
7
[Preoperative molecular staging of colorectal cancers by CM10 ProteinChip and SELDI-TOF-MS analysis].[应用CM10蛋白质芯片和表面增强激光解吸电离飞行时间质谱分析对结直肠癌进行术前分子分期]
Zhonghua Zhong Liu Za Zhi. 2006 Oct;28(10):753-7.
8
Pancreatic cancer biomarkers discovery by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry.通过表面增强激光解吸电离飞行时间质谱法发现胰腺癌生物标志物
Clin Chem Lab Med. 2009;47(6):713-23. doi: 10.1515/CCLM.2009.158.
9
Surface-enhanced laser desorption/ionization time-of-flight proteomic profiling of breast carcinomas identifies clinicopathologically relevant groups of patients similar to previously defined clusters from cDNA expression.乳腺癌的表面增强激光解吸/电离飞行时间蛋白质组分析可识别出临床病理相关的患者群体，这些群体与先前通过cDNA表达定义的聚类相似。
Breast Cancer Res. 2008;10(3):R48. doi: 10.1186/bcr2101. Epub 2008 May 29.
10
Study of distinct serum proteomics for the biomarkers discovery in colorectal cancer.用于发现结直肠癌生物标志物的独特血清蛋白质组学研究。
Discov Med. 2015 Oct;20(110):239-53.

引用本文的文献

1
Circulating Biomarkers of Colorectal Cancer (CRC)-Their Utility in Diagnosis and Prognosis.结直肠癌（CRC）的循环生物标志物——它们在诊断和预后中的应用
J Clin Med. 2021 May 28;10(11):2391. doi: 10.3390/jcm10112391.
2
Proteomic Profiling of Serial Prediagnostic Serum Samples for Early Detection of Colon Cancer in the U.S. Military.美国军队中用于结肠癌早期检测的系列诊断前血清样本的蛋白质组学分析
Cancer Epidemiol Biomarkers Prev. 2017 May;26(5):711-718. doi: 10.1158/1055-9965.EPI-16-0732. Epub 2016 Dec 21.
3
Colorectal cancer: from prevention to personalized medicine.

本文引用的文献

1
the need for review and understanding of SELDI/MALDI mass spectroscopy data prior to analysis.在分析之前对表面增强激光解吸电离/基质辅助激光解吸电离质谱数据进行审查和理解的必要性。
Cancer Inform. 2005;1(1):86-97.
2
Stool DNA and occult blood testing for screen detection of colorectal neoplasia.用于结直肠肿瘤筛查检测的粪便DNA和潜血检测
Ann Intern Med. 2008 Oct 7;149(7):441-50, W81. doi: 10.7326/0003-4819-149-7-200810070-00004.
3
A simplified, noninvasive stool DNA test for colorectal cancer detection.一种用于检测结直肠癌的简化、非侵入性粪便DNA检测方法。
结直肠癌：从预防到个性化医疗
World J Gastroenterol. 2014 Jun 14;20(22):6786-808. doi: 10.3748/wjg.v20.i22.6786.
4
Identification alpha-2-HS-glycoprotein precursor and tubulin beta chain as serology diagnosis biomarker of colorectal cancer.鉴定α-2-HS-糖蛋白前体和微管蛋白β链作为结直肠癌的血清学诊断生物标志物。
Diagn Pathol. 2014 Mar 12;9:53. doi: 10.1186/1746-1596-9-53.
5
Proteomic approaches in biomarker discovery: new perspectives in cancer diagnostics.蛋白质组学方法在生物标志物发现中的应用：癌症诊断的新视角
ScientificWorldJournal. 2014 Jan 14;2014:260348. doi: 10.1155/2014/260348. eCollection 2014.
6
Screening for colorectal cancer: established and emerging modalities.结直肠癌筛查：已确立和新兴方法。
Nat Rev Gastroenterol Hepatol. 2011 Nov 1;8(12):711-22. doi: 10.1038/nrgastro.2011.205.
7
Serum tests for colorectal cancer screening.结直肠癌筛查的血清学检测。
Mol Diagn Ther. 2011 Jun 1;15(3):129-41. doi: 10.1007/BF03256403.
8
A comprehensive peptidome profiling technology for the identification of early detection biomarkers for lung adenocarcinoma.一种全面的肽组学分析技术，用于鉴定肺腺癌的早期检测生物标志物。
PLoS One. 2011 Apr 12;6(4):e18567. doi: 10.1371/journal.pone.0018567.
9
Proteomic serum biomarkers and their potential application in cancer screening programs.蛋白质组学血清生物标志物及其在癌症筛查计划中的潜在应用。
Int J Mol Sci. 2010 Oct 26;11(11):4175-93. doi: 10.3390/ijms11114175.
10
Proteomic strategies and challenges in tumor metastasis research.蛋白质组学策略及其在肿瘤转移研究中的挑战。
Clin Exp Metastasis. 2010 Aug;27(6):441-51. doi: 10.1007/s10585-010-9339-7. Epub 2010 Jul 7.
Am J Gastroenterol. 2008 Nov;103(11):2862-70. doi: 10.1111/j.1572-0241.2008.02088.x. Epub 2008 Aug 27.
4
SELDI-TOF MS whole serum proteomic profiling with IMAC surface does not reliably detect prostate cancer.使用IMAC表面的SELDI-TOF MS全血清蛋白质组分析不能可靠地检测前列腺癌。
Clin Chem. 2008 Jan;54(1):53-60. doi: 10.1373/clinchem.2007.091496. Epub 2007 Nov 16.
5
Analytical validation of serum proteomic profiling for diagnosis of prostate cancer: sources of sample bias.血清蛋白质组分析用于前列腺癌诊断的分析验证：样本偏差来源
Clin Chem. 2008 Jan;54(1):44-52. doi: 10.1373/clinchem.2007.091470. Epub 2007 Nov 2.
6
Diagnosis of esophageal adenocarcinoma by serum proteomic pattern.通过血清蛋白质组图谱诊断食管腺癌。
Ann Thorac Surg. 2007 Aug;84(2):384-92; discussion 392. doi: 10.1016/j.athoracsur.2007.03.088.
7
Preanalytic influence of sample handling on SELDI-TOF serum protein profiles.样本处理对表面增强激光解吸电离飞行时间质谱血清蛋白谱的分析前影响。
Clin Chem. 2007 Apr;53(4):645-56. doi: 10.1373/clinchem.2006.080101. Epub 2007 Feb 15.
8
A serum proteomic pattern for the detection of colorectal adenocarcinoma using surface enhanced laser desorption and ionization mass spectrometry.一种使用表面增强激光解吸电离质谱法检测结肠直肠癌的血清蛋白质组模式。
Cancer Invest. 2006 Dec;24(8):747-53. doi: 10.1080/07357900601063873.
9
Identification of serum biomarkers for colon cancer by proteomic analysis.通过蛋白质组学分析鉴定结肠癌的血清生物标志物
Br J Cancer. 2006 Jun 19;94(12):1898-905. doi: 10.1038/sj.bjc.6603188. Epub 2006 Jun 6.
10
Changes in the serum proteome associated with the development of hepatocellular carcinoma in hepatitis C-related cirrhosis.与丙型肝炎相关肝硬化中肝细胞癌发生发展相关的血清蛋白质组变化
Br J Cancer. 2006 Jan 30;94(2):287-92. doi: 10.1038/sj.bjc.6602923.