Martin M L, Regan C M
Department of Pharmacology, University College, Dublin, Ireland.
Toxicol Lett. 1991 Dec;59(1-3):197-202. doi: 10.1016/0378-4274(91)90072-e.
Synchronous populations of rat C6 glioma cells have been obtained by a mitotic selection procedure and used to establish the effect of transient temperature elevation on their progression through the cell cycle. A transient 3-h exposure to a 42 degree C heat shock within defined area in the mid-G1-phase of the cell cycle has been demonstrated to impair progression to S-phase. This anti-proliferative heat shock effect was not apparent in asynchronous cell populations. A small, but significant, decrease in 3H-thymidine incorporation was observed (approximately 5%) following an 8-h exposure to heat shock.
通过有丝分裂选择程序获得了大鼠C6胶质瘤细胞的同步群体，并用于确定短暂温度升高对其细胞周期进程的影响。已证明在细胞周期的G1期中期的特定区域内短暂暴露于42℃热休克3小时会损害向S期的进程。这种抗增殖热休克效应在非同步细胞群体中不明显。在暴露于热休克8小时后，观察到3H-胸腺嘧啶核苷掺入量有小幅但显著的下降（约5%）。
