Dewey D L, Holden J L
Cell Tissue Kinet. 1984 Jul;17(4):345-50. doi: 10.1111/j.1365-2184.1984.tb00594.x.
Synchronized cells of the Harding Passey melanoma grown in culture were given a heat shock treatment of 44 degrees C for 36 min. Thymidine incorporation was measured at frequent intervals after heat shock to determine the time of onset of the next DNA synthetic period. If the heat shock was given at the end of G1, the following S was delayed by 20 hr. Heating at other times in the cell cycle resulted in an even longer interval before the onset of S. The end of G1 was also the most resistant to hyperthermic killing and to the effect of heat on the magnitude of thymidine incorporation in the following S. Heating the cells a second time did not repeat the effect of the first treatment unless the second heat shock treatment was at a considerably higher temperature. Thus thermotolerance to heat shock killing also applies to cell-cycle delay.
对培养的哈丁·帕西黑色素瘤同步化细胞进行44摄氏度、持续36分钟的热休克处理。热休克后频繁测定胸苷掺入情况，以确定下一个DNA合成期开始的时间。如果在G1期结束时给予热休克，随后的S期会延迟20小时。在细胞周期的其他时间加热，会导致S期开始前的间隔更长。G1期结束时对高温杀伤以及热对随后S期胸苷掺入量的影响也最具抗性。除非第二次热休克处理的温度显著更高，否则再次加热细胞不会重复第一次处理的效果。因此，对热休克杀伤的热耐受性也适用于细胞周期延迟。
