Zettergren L D, Conrad C A, Petering D H, Weber D N, Goodrich M S
Department of Biology, Carroll College, Waukesha, WI 53186.
Toxicol Lett. 1991 Dec;59(1-3):221-8. doi: 10.1016/0378-4274(91)90075-h.
Previous observations suggested that Rana tadpoles treated with aqueous cadmium (Cd) accumulate Cd in their liver and mesonephros. In order to study the response to Cd in these tissues we (a) exposed tadpoles in mid-limb bud stages to sublethal quantities of Cd, (b) isolated Cd-associated protein (CAP) from a liver cytosol fraction, (c) prepared a heterologous rabbit antiserum against glutaraldehyde-treated CAP (G-CAP), (d) used the rabbit anti-G-CAP antiserum in order to assess the tissue distribution of CAP in Cd-treated and untreated tadpoles, and (e) assessed species cross-reactivities of our anti-G-CAP with CAPs and metallothioneins (MTs) isolated from Cd-treated vertebrate liver cytosol fractions. We found that (a) CAP was present in higher quantities in liver cytosol obtained from Cd-treated tadpoles compared to liver cytosol obtained from untreated control tadpoles, (b) indirect immunofluorescent analysis revealed that CAP was localized in liver hepatocytes and kidney tubule epithelial cells in Cd-treated tadpoles, and (c) the anti-G-CAP crossreacted with rodent and fish CAP. These observations suggest that the developing liver and mesonephros are involved in responses to toxic metals and that our anti G-CAP antiserum may be used to gauge exposure to environmental Cd.
先前的观察表明，用镉（Cd）水溶液处理的林蛙蝌蚪会在其肝脏和中肾中积累镉。为了研究这些组织对镉的反应，我们（a）将处于肢体芽中期的蝌蚪暴露于亚致死量的镉中，（b）从肝脏胞质溶胶组分中分离出镉相关蛋白（CAP），（c）制备针对戊二醛处理的CAP（G-CAP）的异源兔抗血清，（d）使用兔抗G-CAP抗血清来评估CAP在镉处理和未处理的蝌蚪中的组织分布，以及（e）评估我们的抗G-CAP与从镉处理的脊椎动物肝脏胞质溶胶组分中分离出的CAP和金属硫蛋白（MT）的种间交叉反应性。我们发现，（a）与从未处理的对照蝌蚪获得的肝脏胞质溶胶相比，从镉处理的蝌蚪获得的肝脏胞质溶胶中CAP的含量更高，（b）间接免疫荧光分析显示，在镉处理的蝌蚪中，CAP定位于肝脏肝细胞和肾小管上皮细胞中，并且（c）抗G-CAP与啮齿动物和鱼类的CAP发生交叉反应。这些观察结果表明，发育中的肝脏和中肾参与了对有毒金属的反应，并且我们的抗G-CAP抗血清可用于衡量对环境镉的暴露情况。
