College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, People's Republic of China.
Environ Sci Pollut Res Int. 2023 May;30(23):64285-64299. doi: 10.1007/s11356-023-26853-1. Epub 2023 Apr 17.
Skin microbiotas play a crucial role in the health, homeostasis, and immune function of amphibians. The contaminants in water could affect the structure and composition of microbial communities. The effects of coexisting pollutants on frogs cannot be adequately explained by a single exposure due to the coexistence of Cd and DEHP in the environment. Following exposure to Cd and/or DEHP, we examined the histological characteristics of Rana chensinensis tadpoles. We also used the 16S rRNA gene sequencing technique to assess the relative abundance of skin microbial communities among tadpoles from each treatment group. Our findings indicate that R. chensinensis' skin experienced some degree of injury due to exposure to Cd and DEHP, which led to the imbalance of their skin microbial community homeostasis and thus interfered with the normal trial status of the host. That may eventually lead to the decline of the amphibian population.
皮肤微生物群在两栖动物的健康、体内平衡和免疫功能中起着至关重要的作用。水中的污染物会影响微生物群落的结构和组成。由于环境中镉和 DEHP 的共存，共存污染物对青蛙的影响不能仅通过单一暴露来充分解释。在暴露于 Cd 和/或 DEHP 后，我们检查了中国林蛙蝌蚪的组织学特征。我们还使用 16S rRNA 基因测序技术评估了来自每个处理组的蝌蚪皮肤微生物群落的相对丰度。我们的研究结果表明，Cd 和 DEHP 的暴露使中国林蛙的皮肤受到了一定程度的损伤，导致其皮肤微生物群落的内稳态失衡，从而干扰了宿主的正常发育状态。这可能最终导致两栖动物种群数量的下降。
