来自食酸戴尔福特菌H16的异源二聚体硝酸还原酶（NapAB）：纯化、结晶及初步X射线分析。
Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis.
作者信息
Coelho Catarina, González Pablo J, Trincão José, Carvalho Ana L, Najmudin Shabir, Hettman Thomas, Dieckman Stephan, Moura José J G, Moura Isabel, Romão Maria J
机构信息
REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):516-9. doi: 10.1107/S1744309107022129. Epub 2007 May 12.
The periplasmic nitrate reductase from Cupriavidus necator (also known as Ralstonia eutropha) is a heterodimer that is able to reduce nitrate to nitrite. It comprises a 91 kDa catalytic subunit (NapA) and a 17 kDa subunit (NapB) that is involved in electron transfer. The larger subunit contains a molybdenum active site with a bis-molybdopterin guanine dinucleotide cofactor as well as one [4Fe-4S] cluster, while the small subunit is a di-haem c-type cytochrome. Crystals of the oxidized form of this enzyme were obtained using polyethylene glycol 3350 as precipitant. A single crystal grown at the High Throughput Crystallization Laboratory of the EMBL in Grenoble diffracted to beyond 1.5 A at the ESRF (ID14-1), which is the highest resolution reported to date for a nitrate reductase. The unit-cell parameters are a = 142.2, b = 82.4, c = 96.8 A, beta = 100.7 degrees, space group C2, and one heterodimer is present per asymmetric unit.
来自食酸铜绿假单胞菌（也称为嗜麦芽寡养单胞菌）的周质硝酸还原酶是一种异源二聚体，能够将硝酸盐还原为亚硝酸盐。它由一个91 kDa的催化亚基（NapA）和一个参与电子转移的17 kDa亚基（NapB）组成。较大的亚基包含一个钼活性位点，带有双钼蝶呤鸟嘌呤二核苷酸辅因子以及一个[4Fe-4S]簇，而小亚基是一种双血红素c型细胞色素。使用聚乙二醇3350作为沉淀剂获得了该酶氧化形式的晶体。在格勒诺布尔欧洲分子生物学实验室的高通量结晶实验室生长的单晶在欧洲同步辐射装置（ID14-1）处衍射分辨率超过1.5 Å，这是迄今为止报道的硝酸盐还原酶的最高分辨率。晶胞参数为a = 142.2，b = 82.4，c = 96.8 Å，β = 100.7°，空间群为C2，每个不对称单元中有一个异源二聚体。
Zotero 插件