[氯乙烯单体所致染色体损伤易感性与APE1、XRCC1基因多态性的相关性研究]

[Study of susceptibility of chromosomal damage induced by vinyl chloride monomer associated with genetic polymorphism in APE1, XRCC1].

作者信息

Qiu Yulan, Zhu Shoumin, Liu Jing, Xia Zhaolin

机构信息

Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Wei Sheng Yan Jiu. 2007 Mar;36(2):132-6.

PMID:17555083

Abstract

OBJECTIVE

To explore the relationship between genetic polymorphism of APE1, XRCC1 and susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM).

METHODS

75 workers occupationally exposed to VCM were investigated. Chromosome damage in peripheral lymphocyte were measured by cytokinesis-block micronucleus (CB-MN) test. Created restriction site-restrained fragment length polymorphism (CRS-RFLP) and PCR-RFLP technique was applied to detect single nucleotide polymorphisms (SNPs) in APE1 gene (c.148) and XRCC1 gene (c. 194, c.280 and c. 399).

RESULTS

The frequencies of the Asp and Glu alleles of APE1 148 in our study population were 61% and 39%, respectively. The frequencies of occurrence of the Arg and Trp alleles of XRCC1 194 were 85% and 15%. The frequencies of occurrence of the Arg and His alleles of XRCC1 280 were 85% and 15%, too. Whereas the prevalence of Arg allele was 87.0% and XRCC1 399 allele Gln was 13.0%. The risk of chromosomal damage induced by VCM for individuals carrying XRCC1 c. 194 Arg/Arg genotype was 0.6898 (95% CI 0.4997 - 0.9333, P = 0.0195) of those carrying Arg/Trp, Trp/Trp genotypes. The risk of chromosomal damage for female VCM exposure workers was 1.6000 (95% CI 1.2284 - 2.0699, P = 0.0004) of male workers. The haplotypes TGG/CGG and TGG/CAGCGGG were associated with the decreased frequencies of total micronucleus ( P < 0. 05).

CONCLUSION

It was suggested that female workers and subjects carrying XRCC1 194 Arg/Arg genotypes could be higher risk of chromosomal damage when they exposed to VCM.

摘要

目的

探讨猿猴病毒1（APE1）、X射线修复交叉互补蛋白1（XRCC1）基因多态性与氯乙烯单体（VCM）所致染色体损伤易感性之间的关系。

方法

对75名职业性接触VCM的工人进行调查。采用胞质分裂阻滞微核（CB-MN）试验检测外周血淋巴细胞染色体损伤情况。应用创建限制位点-限制性片段长度多态性（CRS-RFLP）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测APE1基因（c.148）和XRCC1基因（c.194、c.280和c.399）的单核苷酸多态性（SNP）。

结果

本研究人群中APE1 148位点的天冬氨酸（Asp）和谷氨酸（Glu）等位基因频率分别为61%和39%。XRCC1 194位点的精氨酸（Arg）和色氨酸（Trp）等位基因频率分别为85%和15%。XRCC1 280位点的Arg和组氨酸（His）等位基因频率也分别为85%和15%。而XRCC1 399位点的Arg等位基因频率为87.0%，谷氨酰胺（Gln）等位基因频率为13.0%。携带XRCC1 c.194 Arg/Arg基因型个体发生VCM所致染色体损伤的风险是携带Arg/Trp、Trp/Trp基因型个体的0.6898（95%可信区间0.4997 - 0.9333，P = 0.0195）。职业接触VCM的女性工人发生染色体损伤的风险是男性工人的1.6000（95%可信区间1.2284 - 2.0699，P = 0.0004）。单倍型TGG/CGG和TGG/CAGCGGG与总微核频率降低相关（P < 0.05）。