中国工人人群中代谢酶的遗传多态性与氯乙烯单体诱导的染色体损伤易感性。
Genetic polymorphisms in metabolizing enzymes and susceptibility of chromosomal damage induced by vinyl chloride monomer in a Chinese worker population.
机构信息
Department of Occupational Health and Toxicology, School of Public Health, Fudan University, China.
出版信息
J Occup Environ Med. 2010 Feb;52(2):163-8. doi: 10.1097/JOM.0b013e3181cac00b.
OBJECTIVE
To evaluate whether polymorphisms in metabolizing enzymes contributed to susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM).
METHODS
Cytokinesis block micronucleus test was performed on 185 VCM-exposed workers and 41 control subjects to detect chromosomal damage in peripheral lymphocytes. The polymerase chain reaction and restriction fragment length polymorphism technique was applied to detect polymorphisms of GSTT1, GSTM1, GSTP1G/A, CYP2E1G/C, and CYP2D6G/C. Poisson regression analysis was performed.
RESULTS
Sex, age, VCM exposure, GSTP1, and CYP2E1 genotype can influence chromosomal damage. There was a 1.51-fold increased micronucleus frequency for GSTP1GG genotypes individuals compared with those GSTP1AA/GA genotype individuals (P < 0.05), the effect of polymorphism in CYP2E1 gene was more pronounced for allele C compared with allele G (P < 0.05).
CONCLUSIONS
Polymorphisms of GSTP1G/A and CYP2E1G/C, which are potential susceptibility biomarkers of chromosomal damage in VCM-exposed worker.
目的
评估代谢酶的多态性是否与氯乙烯单体（VCM）诱导的染色体损伤易感性有关。
方法
采用胞质阻滞微核试验检测 185 名 VCM 暴露工人和 41 名对照者外周血淋巴细胞的染色体损伤情况。应用聚合酶链反应和限制性片段长度多态性技术检测 GSTT1、GSTM1、GSTP1G/A、CYP2E1G/C 和 CYP2D6G/C 的多态性。采用泊松回归分析。
结果
性别、年龄、VCM 暴露、GSTP1 和 CYP2E1 基因型均能影响染色体损伤。与 GSTP1AA/GA 基因型个体相比，GSTP1GG 基因型个体的微核频率增加了 1.51 倍（P<0.05），CYP2E1 基因的多态性对等位基因 C 的影响比等位基因 G 更为显著（P<0.05）。
结论
GSTP1G/A 和 CYP2E1G/C 多态性是 VCM 暴露工人染色体损伤的潜在易感性生物标志物。
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