一种自我氨酰化核酶的效率：其3' 延伸序列的长度和碱基组成的影响
Efficiency of a self-aminoacylating ribozyme: effect of the length and base-composition of its 3' extension.
作者信息
Lehmann Jean, Reichel Amy, Buguin Axel, Libchaber Albert
机构信息
Center for Studies in Physics and Biology, The Rockefeller University, New York, NY, 10021, USA.
出版信息
RNA. 2007 Aug;13(8):1191-7. doi: 10.1261/rna.500907. Epub 2007 Jun 7.
Abstract
Variants of a previously described small self-aminoacylating ribozyme are tested in order to uncover the potentialities of a 3' extension responsible for the esterification. The base-composition and the length of this specific part of the ribozyme are investigated. Very short extensions can still reach the active site, reflecting the small persistence length of RNA. The yield of aminoacylation is particularly high for ribozymes with extensions made up of a poly-U, for which a maximum of efficiency is observed for a total length of about 10 nucleotides. A simple model describing the behavior of this region of the ribozyme can account for the data.
摘要
为了揭示负责酯化作用的3'端延伸部分的潜力，对先前描述的一种小型自我氨酰化核酶的变体进行了测试。研究了该核酶这一特定部分的碱基组成和长度。非常短的延伸部分仍能到达活性位点，这反映了RNA较小的持久长度。对于由聚-U组成延伸部分的核酶，氨酰化产率特别高，在总长度约为10个核苷酸时观察到了最大效率。一个描述该核酶这一区域行为的简单模型可以解释这些数据。
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