2005/2006年德国的A组轮状病毒基因型

Group A rotavirus genotypes in Germany during 2005/2006.

作者信息

Mas Marques A, Diedrich S, Huth C, Schreier E

机构信息

Department of Molecular Epidemiology of Viral Pathogens, Robert Koch-Institute, Berlin, Germany.

出版信息

Arch Virol. 2007;152(9):1743-9. doi: 10.1007/s00705-007-0998-x. Epub 2007 Jun 8.

DOI:10.1007/s00705-007-0998-x

PMID:17557132

Abstract

During the 2005/2006 winter season a total of 802 group A positive rotavirus specimens of patients from different regions throughout Germany were genotyped. Amplicons from a one-tube RT-PCR were typed by analysis of their (type-specific) size using type-specific primers, fluorescent consensus primers and a capillary sequencer for detection. While G1P[8] was predominant (45.8%), G9P[8] has emerged as the second most frequent genotype combination (37.7%). The distribution of genotypes was heterogeneous, regional frequencies regarding G1 and G9 were ranging from 15.0 to 89.3% and from 7.1 to 67.7%, respectively. Furthermore, a few human rotavirus G10P[6] and G10P[8] infections were observed.

摘要

在2005/2006年冬季，对来自德国各地不同地区的802份A组阳性轮状病毒患者样本进行了基因分型。通过使用型特异性引物、荧光通用引物和毛细管测序仪分析单管逆转录聚合酶链反应（RT-PCR）扩增产物的（型特异性）大小来进行分型检测。虽然G1P[8]占主导地位（45.8%），但G9P[8]已成为第二常见的基因型组合（37.7%）。基因型分布不均一，G1和G9的区域频率分别在15.0%至89.3%和7.1%至67.7%之间。此外，还观察到了几例人轮状病毒G10P[6]和G10P[8]感染。