Le Van Phan, Kim Ji-Yeon, Cho Sung-Lim, Nam Sun-Woo, Lim Inseok, Lee Hee-Joong, Kim Kijeong, Chung Sang-In, Song Wonkeun, Lee Kyu Man, Rhee Moon-Soo, Lee Jung-Sook, Kim Wonyong
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
J Med Virol. 2008 Jan;80(1):175-82. doi: 10.1002/jmv.21044.
Five hundred four fecal specimens, collected between 2004 and 2006 from young children with acute diarrhea, were screened for rotavirus by ELISA with VP6-specific antibody. Of these samples, 394 (78.2%) were confirmed as group A rotavirus and they underwent G- and P typing using a combination of ELISA, RT-PCR, and sequence analysis methods. The dominant circulating G serotype was G1 (35.6%) followed by G3 (26.4%), G4 (14.7%), and G2 (11.9%). There was a low prevalence of G9 (1.0%) and of unusual G type rotavirus, in particular, G12 (0.5%) and G8 (0.3%). Of the P genotype rotavirus in circulation, P[8] (53.0%) was most common followed by P[6] (15.5%), P[4] (15.2%), and P[9] (2.3%). Determination of G- and P type combinations revealed that G1P[8] strains were most prevalent (25.4%), amid G3P[8] (16.8%), G2P[4] (6.3%), and G4P[6] (6.1%) strains. Unusual or rare combinations such as G2P[6], G2P[8], G3P[4], G2P[9], G1P[9], G3P[9], G12P[6], G1P[4], G3P[6], and G8P[8] were also found. Owing to the recent emergence of G8 and G12 rotavirus, the findings from this study are important since they provide new information concerning the local and global spread of rotavirus genotypes.
2004年至2006年间，收集了504份来自患有急性腹泻的幼儿的粪便标本，采用针对VP6的特异性抗体的ELISA法对轮状病毒进行筛查。在这些样本中，394份（78.2%）被确认为A组轮状病毒，并采用ELISA、RT-PCR和序列分析方法相结合的方式对其进行G型和P型分型。主要流行的G血清型为G1（35.6%），其次是G3（26.4%）、G4（14.7%）和G2（11.9%）。G9（1.0%）以及不常见的G型轮状病毒，尤其是G12（0.5%）和G8（0.3%）的流行率较低。在流行的P基因型轮状病毒中，P[8]（53.0%）最为常见，其次是P[6]（15.5%）、P[4]（15.2%）和P[9]（2.3%）。G型和P型组合的测定显示，G1P[8]毒株最为普遍（25.4%），其次是G3P[8]（16.8%）、G2P[4]（6.3%）和G4P[6]（6.1%）毒株。还发现了不常见或罕见的组合，如G2P[6]、G2P[8]、G3P[4]、G2P[9]、G1P[9]、G3P[9]、G12P[6]、G1P[4]、G3P[6]和G8P[8]。由于最近出现了G8和G12轮状病毒，本研究的结果具有重要意义，因为它们提供了有关轮状病毒基因型在本地和全球传播的新信息。
