一种基于微型计算机断层扫描的方法，用于测量健康小鼠和博来霉素处理小鼠的肺顺应性。
A micro-computed tomography-based method for the measurement of pulmonary compliance in healthy and bleomycin-exposed mice.
作者信息
Shofer Scott, Badea Cristian, Auerbach Scott, Schwartz David A, Johnson G Allan
机构信息
Pulmonary, Allergy, and Critical Care, Duke University Medical Center, Durham, North Carolina 27710, USA.
出版信息
Exp Lung Res. 2007 Apr-May;33(3-4):169-83. doi: 10.1080/01902140701364458.
Abstract
Micro-computed tomography (microCT) is being increasingly used to examine small animal models of pulmonary injury. The authors have developed a microCT technique suitable for the determination of pulmonary compliance in injured mice. Lung volumes in normal mice were radiographically determined at end-inspiration and end-expiration and pulmonary compliance was calculated at 2 time points 2 weeks apart, whereas a second group of mice were given bleomycin and imaged 3 weeks following drug administration. Compliance measurements were validated using a commercially available ventilator system. MicroCT pulmonary compliance measurements are suitable for longitudinal measurements, and correlate with physiologic measurements of pulmonary compliance.
摘要
微型计算机断层扫描（microCT）越来越多地用于检查肺部损伤的小动物模型。作者开发了一种适用于测定受伤小鼠肺顺应性的microCT技术。在吸气末和呼气末通过X射线测定正常小鼠的肺容积，并在相隔2周的2个时间点计算肺顺应性，而第二组小鼠给予博来霉素，并在给药后3周进行成像。使用市售的通气系统对顺应性测量进行验证。MicroCT肺顺应性测量适用于纵向测量，并且与肺顺应性的生理测量相关。
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