肺部疾病小动物成像模型的成像技术：微型计算机断层扫描
Imaging techniques for small animal imaging models of pulmonary disease: micro-CT.
作者信息
Johnson Kennita A
机构信息
Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
出版信息
Toxicol Pathol. 2007 Jan;35(1):59-64. doi: 10.1080/01926230601184262.
Abstract
Microcomputed tomography (micro-CT) is ideal for quantifying pulmonary disease because of the inherent contrast between tissue and air that exists in the lungs. Both in vivo and in vitro studies can be performed using micro-CT. Live animal studies show function, while fixed specimen studies show structure. Through the use of image processing techniques, both acute and chronic lung diseases can be quantified. The information provided by micro-CT is complementary to histological evaluation, since CT is nondestructive. This paper discusses two examples, in vivo and in vitro, of how micro-CT can be used to assess pulmonary diseases in small animal models. With the use of micro-CT, we were able to quantify pulmonary fibrosis in the live rat and investigate the microstructure of the airway in fixed mouse lungs.
摘要
微计算机断层扫描（micro-CT）由于肺部组织与空气之间存在固有的对比度，因此非常适合对肺部疾病进行量化。体内和体外研究均可使用微计算机断层扫描进行。活体动物研究显示功能，而固定标本研究显示结构。通过使用图像处理技术，急性和慢性肺部疾病均可进行量化。微计算机断层扫描提供的信息与组织学评估互补，因为计算机断层扫描是无损的。本文讨论了两个例子，体内和体外的，说明微计算机断层扫描如何用于评估小动物模型中的肺部疾病。通过使用微计算机断层扫描，我们能够量化活鼠的肺纤维化，并研究固定小鼠肺中气道的微观结构。
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本文引用的文献
1
Three-dimensional segmentation and skeletonization to build an airway tree data structure for small animals.用于构建小动物气道树数据结构的三维分割与骨架化
Phys Med Biol. 2005 Apr 7;50(7):1405-19. doi: 10.1088/0031-9155/50/7/005. Epub 2005 Mar 16.
2
Micro-CT with respiratory and cardiac gating.带有呼吸和心脏门控的微型计算机断层扫描
Med Phys. 2004 Dec;31(12):3324-9. doi: 10.1118/1.1812604.
3
Improved method of in vivo respiratory-gated micro-CT imaging.体内呼吸门控微型计算机断层扫描成像的改进方法
Phys Med Biol. 2004 Sep 7;49(17):4163-72. doi: 10.1088/0031-9155/49/17/023.
4
In vivo respiratory-gated micro-CT imaging in small-animal oncology models.小动物肿瘤模型中的体内呼吸门控微型计算机断层扫描成像
Mol Imaging. 2004 Jan;3(1):55-62. doi: 10.1162/15353500200403184.
5
Recent advances in imaging the lungs of intact small animals.完整小动物肺部成像的最新进展。
Am J Respir Cell Mol Biol. 2004 Feb;30(2):129-38. doi: 10.1165/rcmb.2003-0213TR.
6
Fundamental image quality limits for microcomputed tomography in small animals.小动物微型计算机断层扫描的基本图像质量限制
Med Phys. 2003 Nov;30(11):2869-77. doi: 10.1118/1.1617353.
7
Characterization of the interstitial lung diseases via density-based and texture-based analysis of computed tomography images of lung structure and function.通过基于密度和纹理的肺结构与功能计算机断层扫描图像分析对间质性肺疾病进行特征描述。
Acad Radiol. 2003 Oct;10(10):1104-18. doi: 10.1016/s1076-6332(03)00330-1.
8
Three-dimensional visualization and morphometry of small airways from microfocal X-ray computed tomography.基于微焦点X射线计算机断层扫描的小气道三维可视化与形态测量
J Biomech. 2003 Nov;36(11):1587-94. doi: 10.1016/s0021-9290(03)00179-9.
9
Molecular imaging in small animals--roles for micro-CT.小动物分子成像——微型计算机断层扫描的作用
J Cell Biochem Suppl. 2002;39:116-24. doi: 10.1002/jcb.10415.
10
A lung computed tomographic assessment of positive end-expiratory pressure-induced lung overdistension.呼气末正压诱导的肺过度膨胀的肺部计算机断层扫描评估
Am J Respir Crit Care Med. 1998 Nov;158(5 Pt 1):1571-7. doi: 10.1164/ajrccm.158.5.9802101.
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