Villanueva-García Dina, Wang Karen T, Nielsen Heber C, Ramadurai Sujatha M
Division of Neonatology, Hospital Infantil de México Federico Gómez, Mexico City, Mexico.
Exp Lung Res. 2007 Apr-May;33(3-4):185-96. doi: 10.1080/01902140701385073.
Epidermal growth factor receptor signaling plays an important role in lung maturation. The authors hypothesized that specific protein kinase C (PKC) isoforms are expressed and activated by epidermal growth factor (EGF) and transforming growth factor alpha (TGFalpha) (EGF receptor [EGFR] ligands) in fetal lung fibroblasts. The authors found 4 PKC isoforms expressed in gestational day 19 (d19) fetal rat lung fibroblasts, and focused on PKCalpha because of its developmental expression pattern. PKCalpha immunolocalization in d17, d19, and d21 fetal lung fibroblasts was similar to EGFR. PKCalpha expression decreased with lung maturation. EGF, but not TGFalpha, stimulated PKCalpha activation and membrane translocation. Further studies of PKCalpha functions in fetal lung development are clearly needed.
表皮生长因子受体信号传导在肺成熟过程中起重要作用。作者推测，特定的蛋白激酶C（PKC）亚型在胎儿肺成纤维细胞中由表皮生长因子（EGF）和转化生长因子α（TGFα）（EGF受体[EGFR]配体）表达并激活。作者发现4种PKC亚型在妊娠第19天（d19）的胎鼠肺成纤维细胞中表达，并因其发育表达模式而聚焦于PKCα。d17、d19和d21胎肺成纤维细胞中的PKCα免疫定位与EGFR相似。PKCα表达随肺成熟而降低。EGF而非TGFα刺激PKCα激活和膜转位。显然需要对PKCα在胎儿肺发育中的功能进行进一步研究。
