Emura M, Kano-Tanaka K, Tanaka T, Kojima K, Hanaichi T
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1976 Jan 2;85(1):39-46. doi: 10.1007/BF00308127.
With the aim of expanding knowledge on the pathogenesis of nephroblastomata, an embryological organ culture system (Grobstein, 1956) was tested for its applicability to in vitro carcinogenesis experiments by using murine sarcoma virus (MSV-M) and 3-methylcholanthrene (MCA). Treatment of CBA/H-T6 mouse metanephrogenic mesenchyma with MSV-M at the pretubular stage neither disturbed the glomerulogenesis nor induced rapid malignant transformation. Treatment of the same tissues with MCA considerably inhibited the glomerulogenesis but failed to also reduce rapid malignant transformation. However, one MSV-M, one MCA and two untreated cultures showed malignant transformation after prolonged survival in vitro and produced different histological types of tumours upon transplantation into newborn CBA/H-T6 mice.
为了拓展对肾母细胞瘤发病机制的认识，我们测试了一种胚胎器官培养系统（格罗布斯坦，1956年），看其是否适用于使用鼠肉瘤病毒（MSV-M）和3-甲基胆蒽（MCA）进行的体外致癌实验。在肾小管前期用MSV-M处理CBA/H-T6小鼠后肾间充质，既未干扰肾小球生成，也未诱导快速恶性转化。用MCA处理相同组织则显著抑制了肾小球生成，但也未能降低快速恶性转化。然而，一份MSV-M处理组、一份MCA处理组以及两份未处理的培养物在体外长期存活后出现了恶性转化，并且移植到新生CBA/H-T6小鼠体内后产生了不同组织学类型的肿瘤。
