Jones D B, Moore M
Br J Cancer. 1974 Jan;29(1):21-30. doi: 10.1038/bjc.1974.3.
Antigens associated with cells transformed in vivo by FBJ virus, a wild type murine sarcoma virus (MSV) complex originating from a spontaneously arising osteosarcoma in a CF1 mouse, have been partially characterized by complement fixation (CF). Using rat antisera against antigens specified by Gross leukaemia virus (GLV) the group specific (gs) antigen of C-type RNA murine tumour viruses was demonstrated in FBJ tumours as well as in GLV rat leukaemias, AKR lymphomata and sarcomata induced by MSV-H (Harvey), an MSV isolate of Friend-Moloney-Rauscher (FMR) subgroup specificity. Using mouse antisera against antigens present in FBJ cells the Gross (G) or wild type specificity of FBJ tumours was demonstrated by cross reactivity with antigens expressed on normal AKR lymphoid tissues and leukaemias. These antigens were absent from MSV-H induced sarcomata and in reciprocal tests mouse antisera to MSV-H failed to react with antigens present in FBJ tumour cells. No distinction between cellular and virion antigens expressed by FBJ cells was possible by CF although evidence for a cellular antigen with G specificity was obtained in tests using aged C57B1 antiserum containing a naturally occurring G antibody lacking significant virus neutralizing capacity. However, the likelihood that mouse FBJ antisera contain antibodies to type specific viral envelope antigens (VEA) as well as cellular antigen is discussed.
与FBJ病毒在体内转化的细胞相关的抗原已通过补体结合试验（CF）进行了部分特征鉴定。FBJ病毒是一种野生型鼠肉瘤病毒（MSV）复合体，源自CF1小鼠自发产生的骨肉瘤。使用针对格罗斯白血病病毒（GLV）指定抗原的大鼠抗血清，在FBJ肿瘤以及GLV大鼠白血病、AKR淋巴瘤和由MSV-H（哈维）诱导的肉瘤中证实了C型RNA鼠肿瘤病毒的群特异性（gs）抗原。MSV-H是Friend-Moloney-Rauscher（FMR）亚组特异性的MSV分离株。使用针对FBJ细胞中存在的抗原的小鼠抗血清，通过与正常AKR淋巴组织和白血病中表达的抗原的交叉反应，证实了FBJ肿瘤的格罗斯（G）或野生型特异性。这些抗原在MSV-H诱导的肉瘤中不存在，在反向试验中，针对MSV-H的小鼠抗血清与FBJ肿瘤细胞中存在的抗原不发生反应。通过CF无法区分FBJ细胞表达的细胞抗原和病毒粒子抗原，尽管在使用含有天然存在的G抗体且缺乏显著病毒中和能力的老龄C57B1抗血清进行的试验中获得了具有G特异性的细胞抗原的证据。然而，本文讨论了小鼠FBJ抗血清中可能同时含有针对型特异性病毒包膜抗原（VEA）和细胞抗原的抗体的可能性。
