来自合并或未合并子痫前期的宫内生长受限妊娠的绒毛滋养层细胞的体外分化

In vitro differentiation of villous trophoblasts from pregnancies complicated by intrauterine growth restriction with and without pre-eclampsia.

作者信息

Newhouse S M, Davidge S T, Winkler-Lowen B, Demianczuk N, Guilbert L J

机构信息

Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada.

出版信息

Placenta. 2007 Oct;28(10):999-1003. doi: 10.1016/j.placenta.2007.04.008. Epub 2007 Jun 7.

DOI:10.1016/j.placenta.2007.04.008

PMID:17559930

Abstract

Highly purified (>99.99%) primary villous cytotrophoblasts from uncomplicated pregnancies and pregnancies complicated with intrauterine growth restriction (IUGR) alone, IUGR with pre-eclampsia (IUGR-PE) and PE alone were cultured for 5days and the extent of differentiation into syncytiotrophoblasts measured in terms of syncytialisation and secretion of chorionic gonadotropin (hCG) and placental lactogen (hPL). Three separate phenotypes were observed: (1) normal and IUGR-PE cells showed low syncytialisation and secretion of hCG and hPL, (2) IUGR cells showed the highest levels of syncytialisation and secretion and (3) PE cells showed high syncytialisation but low secretion. These results strongly suggest IUGR, IUGR-PE and PE to be distinct conditions in which villous cytotrophoblasts are either exposed to different environments or are genetically different.

摘要

从无并发症妊娠以及仅合并胎儿生长受限（IUGR）、合并子痫前期的胎儿生长受限（IUGR-PE）和仅患子痫前期（PE）的妊娠中获取高度纯化（>99.99%）的原代绒毛细胞滋养层细胞，将其培养5天，并根据合体化以及绒毛膜促性腺激素（hCG）和胎盘催乳素（hPL）的分泌情况来测定其分化为合体滋养层细胞的程度。观察到三种不同的表型：（1）正常细胞和IUGR-PE细胞显示出较低的合体化以及hCG和hPL分泌水平，（2）IUGR细胞显示出最高水平的合体化和分泌，（3）PE细胞显示出较高的合体化但分泌水平较低。这些结果有力地表明，IUGR、IUGR-PE和PE是不同的情况，其中绒毛细胞滋养层细胞要么暴露于不同的环境，要么在基因上存在差异。