Dagnon Koami, Heudes Didier, Bernaudin Jean-François, Callard Patrice
Service d' Anatomie Pathologique and UPRES EA 3499, Université Paris 6, Hôpital Tenon, 4 Rue de la Chine, Paris, France.
Microvasc Res. 2008 Jan;75(1):112-8. doi: 10.1016/j.mvr.2007.04.004. Epub 2007 Apr 21.
Interactions between tumor cells and microvasculature are particularly critical. This computerized morphometric study was designed to analyze the distance between cancer cells and blood vessels and microvasculature organization in non-small cell lung carcinoma (NSCLC) comparing squamous cell carcinoma (SCC) and adenocarcinoma (ADC). Seventy nine nests of tumor cells, located less than or more than 3 mm from the invading edge, with a similar surface area, and their surrounding stroma were analyzed. After immunolabeling with an antihuman CD34 monoclonal antibody, computerized morphometric analyses of microvascular density (MVD), distribution of microvessels within stroma, and fractions of carcinomatous cells over various distances from microvessels were performed. This analysis showed a significantly higher MVD score in ADC than in SCC, particularly close to the invading edge (382+/-57 in ADC <3 mm; 242+/-28 in SCC <3 mm, p=0.015). Moreover, a significantly higher proportion of cancer cells was situated more than 75 microm from microvessels in SCC than in ADC, regardless of their site in relation to the invading edge (for example, 25+/-5% in ADC <3 mm; 52+/-3% in SCC <3 mm, p=0.001).
肿瘤细胞与微血管之间的相互作用尤为关键。本计算机形态计量学研究旨在分析非小细胞肺癌（NSCLC）中癌细胞与血管之间的距离以及微血管组织情况，比较鳞状细胞癌（SCC）和腺癌（ADC）。分析了79个肿瘤细胞巢，这些肿瘤细胞巢位于距浸润边缘小于或大于3毫米处，具有相似的表面积，以及它们周围的基质。在用抗人CD34单克隆抗体进行免疫标记后，对微血管密度（MVD）、基质内微血管分布以及距微血管不同距离处的癌细胞比例进行了计算机形态计量分析。该分析显示，ADC中的MVD评分显著高于SCC，尤其是在靠近浸润边缘处（ADC <3毫米时为382±57；SCC <3毫米时为242±28，p = 0.015）。此外，无论肿瘤细胞巢相对于浸润边缘的位置如何，SCC中距微血管超过75微米的癌细胞比例显著高于ADC（例如，ADC <3毫米时为25±5%；SCC <3毫米时为52±3%，p = 0.001）。
