Liu Fei, Xu Yu, Gao Shu, Zhang Jundong, Guo Qingxiang
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
J Pharm Biomed Anal. 2007 Sep 3;44(5):1187-91. doi: 10.1016/j.jpba.2007.04.020. Epub 2007 Apr 24.
In this study, a fast and sensitive liquid chromatography/tandem mass spectrometry method for determination of hydrochlorothiazide in human plasma was developed and validated. The analyte and irbesartan, used as the internal standard, were precipitated and extracted from plasma using methanol. Analysis was performed on a Phenomenex Kromasil C(8) column with water and methanol (27:73, v/v) as the mobile phase. Linearity was assessed from 0.78 to 200 ng/mL in plasma. The analytical method proved to be applicable in a pharmacokinetic study after oral administration of 12 mg hydrochlorothiazide tablets to 20 healthy volunteers.
本研究建立并验证了一种快速、灵敏的液相色谱/串联质谱法，用于测定人血浆中的氢氯噻嗪。以厄贝沙坦作为内标，将分析物和内标从血浆中用甲醇沉淀并萃取出来。采用Phenomenex Kromasil C(8)柱，以水和甲醇（27:73，v/v）作为流动相进行分析。血浆中线性范围为0.78至200 ng/mL。该分析方法经证明适用于对20名健康志愿者口服12 mg氢氯噻嗪片后的药代动力学研究。
