Shcharbin Dzmitry, Janicka Magdalena, Wasiak Michal, Palecz Bartlomiej, Przybyszewska Magdalena, Zaborski Marian, Bryszewska Maria
Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Biochim Biophys Acta. 2007 Jul;1774(7):946-51. doi: 10.1016/j.bbapap.2007.04.016. Epub 2007 May 6.
The detailed analysis of the interaction between PAMAM G4 dendrimer and serum albumins was performed using circular dichroism, isothermal titration calorimetry, capillary electrophoresis, zeta-potential and fluorescence polarization. It was shown that serum albumins and PAMAM G4 dendrimer form the complex with stoichiometry of 4-6:1 for G4:HSA and 4-5:1 for G4:BSA molar ratio. The possible sites of PAMAM G4 dendrimers binding to protein surface were discussed. Also, it has been proposed that dendrimer does not significantly affect the protein secondary structure studied by circular dichroism.
使用圆二色性、等温滴定量热法、毛细管电泳、zeta电位和荧光偏振对聚酰胺-胺型（PAMAM）四代树枝状大分子与血清白蛋白之间的相互作用进行了详细分析。结果表明，血清白蛋白与PAMAM四代树枝状大分子形成复合物，对于G4:人血清白蛋白（HSA），化学计量比为4 - 6:1，对于G4:牛血清白蛋白（BSA），化学计量比为4 - 5:1（摩尔比）。讨论了PAMAM四代树枝状大分子与蛋白质表面结合的可能位点。此外，有人提出树枝状大分子对通过圆二色性研究的蛋白质二级结构没有显著影响。
