利用针对宿主细胞基因的小干扰RNA（siRNA）微型文库鉴定人类免疫缺陷病毒1型复制的抑制因子。

Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes.

作者信息

Kameoka Masanori, Kitagawa Yukiko, Utachee Piraporn, Jinnopat Piyamat, Dhepakson Panadda, Isarangkura-na-ayuthaya Panasda, Tokunaga Kenzo, Sato Hironori, Komano Jun, Yamamoto Naoki, Oguchi Shinobu, Natori Yukikazu, Ikuta Kazuyoshi

机构信息

Section of Viral infections, Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi 11000, Thailand.

出版信息

Biochem Biophys Res Commun. 2007 Aug 3;359(3):729-34. doi: 10.1016/j.bbrc.2007.05.173. Epub 2007 Jun 4.

DOI:10.1016/j.bbrc.2007.05.173

PMID:17560945

Abstract

We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.

摘要

我们使用由针对细胞基因的257条小干扰RNA（siRNA）组成的小干扰RNA微型文库进行筛选，以寻找影响1型人类免疫缺陷病毒（HIV-1）复制的新型宿主因子。将人急性单核细胞白血病细胞系J111细胞用单个小干扰RNA转染，随后在96孔板中用带有荧光素酶报告基因的HIV-1分子克隆进行感染或转染。结果显示，6条小干扰RNA显著增强了J111细胞中HIV-1的复制，这表明这些小干扰RNA的靶细胞基因在正常细胞培养条件下可能对HIV-1复制起负调控作用。我们还讨论了这些细胞蛋白调节病毒复制的可能机制。