Jonasson Anette, Eriksson Christer, Jenkinson Howard F, Källestål Carina, Johansson Ingegerd, Strömberg Nicklas
Department of Odontology/Cariology, Umeå University, Umeå, Sweden.
BMC Infect Dis. 2007 Jun 11;7:57. doi: 10.1186/1471-2334-7-57.
Bacterial adhesion is an important determinant of colonization and infection, including dental caries. The salivary scavenger receptor cysteine-rich glycoprotein gp-340, which mediates adhesion of Streptococcus mutans (implicated in caries), harbours three major size variants, designated gp-340 I to III, each specific to an individual saliva. Here we have examined the association of the gp-340 I to III polymorphisms with caries experience and adhesion of S. mutans.
A case-referent study was performed in 12-year-old Swedish children with high (n = 19) or low (n = 19) caries experiences. We measured the gp-340 I to III saliva phenotypes and correlated those with multiple outcome measures for caries experience and saliva adhesion of S. mutans using the partial least squares (PLS) multivariate projection technique. In addition, we used traditional statistics and 2-year caries increment to verify the established PLS associations, and bacterial adhesion to purified gp-340 I to III proteins to support possible mechanisms.
All except one subject were typed as gp-340 I to III (10, 23 and 4, respectively). The gp-340 I phenotype correlated positively with caries experience (VIP = 1.37) and saliva adhesion of S. mutans Ingbritt (VIP = 1.47). The gp-340 II and III phenotypes tended to behave in the opposite way. Moreover, the gp-340 I phenotype tended to show an increased 2-year caries increment compared to phenotypes II/III. Purified gp-340 I protein mediated markedly higher adhesion of S. mutans strains Ingbritt and NG8 and Lactococcus lactis expressing AgI/II adhesins (SpaP or PAc) compared to gp-340 II and III proteins. In addition, the gp-340 I protein appeared over represented in subjects positive for Db, an allelic acidic PRP variant associated with caries, and subjects positive for both gp-340 I and Db tended to experience more caries than those negative for both proteins.
Gp-340 I behaves as a caries susceptibility protein.
细菌黏附是包括龋齿在内的定植和感染的重要决定因素。唾液富含半胱氨酸的清道夫受体糖蛋白gp - 340介导变形链球菌（与龋齿相关）的黏附，它有三种主要的大小变体，命名为gp - 340 I至III，每种变体都特异性地存在于个体唾液中。在此，我们研究了gp - 340 I至III多态性与龋齿经历及变形链球菌黏附之间的关联。
对12岁有高（n = 19）或低（n = 19）龋齿经历的瑞典儿童进行病例对照研究。我们测量了gp - 340 I至III唾液表型，并使用偏最小二乘法（PLS）多元投影技术将这些表型与龋齿经历和变形链球菌唾液黏附的多种结果指标进行关联分析。此外，我们使用传统统计学方法和2年龋齿增量来验证已建立的PLS关联，并通过细菌对纯化的gp - 340 I至III蛋白的黏附来支持可能的机制。
除一名受试者外，所有受试者均被分型为gp - 340 I至III（分别为10例、23例和4例）。gp - 340 I表型与龋齿经历（VIP = 1.37）和变形链球菌Ingbritt的唾液黏附（VIP = 1.47）呈正相关。gp - 340 II和III表型的表现则相反。此外，与II/III表型相比，gp - 340 I表型的2年龋齿增量有增加趋势。与gp - 340 II和III蛋白相比，纯化的gp - 340 I蛋白介导变形链球菌Ingbritt菌株、NG8菌株以及表达AgI/II黏附素（SpaP或PAc）的乳酸乳球菌的黏附明显更高。此外，在与龋齿相关的等位基因酸性富脯蛋白变体Db呈阳性的受试者以及gp - 340 I和Db均呈阳性的受试者中，gp - 340 I蛋白的出现频率似乎更高，且这两类受试者往往比两种蛋白均为阴性的受试者患龋齿更多。
Gp - 340 I表现为一种龋齿易感蛋白。
