[菜粉蝶（鳞翅目昆虫）前胸腺中蜕皮甾体的生物合成。3-脱氢蜕皮酮放射性标记前体的体外转化]
[Biosynthesis of ecdysteroids by prothoracic glands in Pieris brassicae (Lepidoptera insect). Conversion in vitro of radiolabelled precursor of 3-dehydroecdysone].
作者信息
Blais C, Lafont R
机构信息
C.N.R.S.-U.R.A. 686, Ecole normale supérieure, Paris.
出版信息
C R Acad Sci III. 1991;313(8):359-64.
Ecdysteroids secreted by prothoracic glands of Pieris brassicae were measured by enzyme immunoassay, after HPLC separation. Both ecdysone and 3-dehydroecdysone, as a major product, are produced. Two radiolabelled putative precursors were converted by Pieris glands in vitro: [3H] ketodiol was converted into ecdysone at a low rate; [3H]3-oxoketodiol was efficiently converted, mainly into 3-dehydroecdysone. These data make 3-oxoketodiol a good candidate for an intermediate in ecdysteroid biosynthetic pathway.
通过高效液相色谱分离后，采用酶免疫分析法测定了粉纹夜蛾前胸腺分泌的蜕皮甾类。结果表明，蜕皮激素和3-脱氢蜕皮激素作为主要产物均有产生。粉纹夜蛾腺体在体外将两种放射性标记的假定前体进行了转化：[3H]酮二醇以较低速率转化为蜕皮激素；[3H]3-氧代酮二醇被高效转化，主要转化为3-脱氢蜕皮激素。这些数据表明3-氧代酮二醇是蜕皮甾类生物合成途径中一种中间体的有力候选物。
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